中医学报2026，Vol.41Issue(5)：1096-1105,10.DOI:10.16368/j.issn.1674-8999.2026.05.163
芪慈三苓汤调控RNF180抑制膀胱癌细胞株T24/DDP顺铂耐药性的作用机制
Mechanism of Qici Sanling Decoction Regulating RNF180 to Inhibit Cisplatin Resistance in Bladder Cancer Cell Line T24/DDP
摘要
Abstract
Objective:To explore the mechanism by which Qici Sanling decoction(QCSL)affects cisplatin resistance in the bladder cancer cell line T24/DDP by regulating ring finger protein 180(RNF180).Methods:Cells were divided into T24 group,T24/DDP group,and T24/DDP QCSL group,with cisplatin concentrations of 0,2.5,5,10 μmol·L-1;cell viability at 0,24,48,and 72 h was measured using CCK-8 assay.T24/DDP cells were divided into Control group,DDP group,QCSL group,and QCSL DDP group;cell apoptosis rate was measured by flow cytometry,and cell invasion and migration were assessed using Transwell assay.qPCR was used to detect RNF180 mRNA expression in bladder cancer patient tumor tissues(Cancer group)and adjacent normal tissues(Normal group).T24/DDP cells were divided into Control group,low-concentration QCSL group(0.5 g·mL-1),medium-concentration QCSL group(1 g·mL-1),and high-concentration QCSL group(2 g·mL-1);RNF180 protein and gene expression levels were de-tected by Western blot and qPCR.T24/DDP cells were divided into Control group,Control DDP group,QCSL DDP group,and QCSL DDP siRNF180 group;RNF180 protein expression levels were measured by Western blot,cell apoptosis rate by flow cytometry,and cell invasion and migration by Transwell assay.Results:CCK-8 assay showed that at 48 h and 72 h of culture,cell proliferation activity de-creased with increasing cisplatin concentration in all groups(P<0.001),and QCSL could significantly improve cisplatin resistance in T24/DDP cells.Compared with the Control group,the apoptosis rate of cells in the DDP group increased(P<0.001);compared with the DDP group and QCSL group,the apoptosis rate of cells in the QCSL DDP group increased(P<0.001).Compared with the Control group,the numbers of cell migration and invasion in the QCSL and DDP groups decreased(P<0.001);compared with the DDP group and QCSL group,the numbers of cell migration and invasion in the QCSL DDP group decreased(P<0.001).Compared with the Nor-mal group,the RNF180 mRNA levels in cancer tissues of patients in the Cancer group were reduced(P<0.01).Compared with the Control group,the RNF180 protein and gene expression levels in the medium and high concentration groups of QCSL increased(P<0.05).Compared with the Control DDP group,RNF180 protein expression levels in the QCSL DDP group and QCSL DDP siRNF180 group increased(P<0.001);compared with the QCSL DDP group,RNF180 protein expression levels in the QCSL DDP siRNF180 group decreased(P<0.001).Compared with the Control group,apoptosis rate increased,and cell migration and invasion decreased in the Control DDP group(P<0.001);compared with the Control DDP group,apoptosis rate increased,and cell migration and invasion decreased in the QCSL DDP group and QCSL DDP siRNF180 group(P<0.001);compared with the QCSL DDP group,apoptosis rate decreased and cell migration and invasion increased in the QCSL DDP siRNF180 group(P<0.001).Conclusion:Qici Sanling Decoc-tion may improve cisplatin resistance in T24/DDP cell by upregulating RNF180 expression.关键词
芪慈三苓汤/膀胱癌/RNF180/顺铂/耐药/T24/DDP 细胞Key words
Qici Sanling Decoction/bladder cancer/RNF180/cisplatin/resistance/T24/DDP cell分类
医药卫生引用本文复制引用
张军,王伟峰,郝继东,廖国强,杨波,龚华..芪慈三苓汤调控RNF180抑制膀胱癌细胞株T24/DDP顺铂耐药性的作用机制[J].中医学报,2026,41(5):1096-1105,10.基金项目
上海市浦东新区卫生健康委员会学科建设项目(PWZzk2022-22) （PWZzk2022-22）
上海健康医学院自然重点项目(SSF-23-17-008) （SSF-23-17-008）
