化工进展2026，Vol.45Issue(4)：2525-2538,14.DOI:10.16085/j.issn.1000-6613.2025-0741
生物炭和铁添加对低温条件下厌氧氨氧化脱氮的影响
Effect of biochar and iron addition on anaerobic ammonia oxidative denitrification under low temperature conditions
摘要
Abstract
To address the current challenges in anaerobic ammonium oxidation(Anammox)processes—Specifically,the accumulation of nitrate byproducts limiting total nitrogen(TN)removal efficiency and the inhibition of nitrogen removal performance at low temperatures—An iron and biochar synergistic strategy for low-temperature enhancement of Anammox coupled with denitrification was proposed in this study.An experiment was conducted using an up-flow Anammox sludge bed.It was operated continuously at room temperature for 159 days.As the temperature decreased,the reactor's nitrogen removal performance was observed to decline continuously.In the 126th day,biochar was introduced into the reactor.Following an adaptation period,the total nitrogen removal rate was gradually increased.To further accelerate the nitrogen removal efficiency,Fe0,Fe2+,and Fe3+were introduced into the reactor in the 144th day.Consequently,the nitrogen removal efficiency was sharply increased from(38.76±3.48)%to(90.81±2.17)%.Concurrently,the nitrate accumulation was reduced from(9.07±1.17)mg/L to(1.79±1.99)mg/L,and the effluent total nitrogen was maintained at(8.78±2.11)mg/L.Based on comprehensive analyses,including SEM,XRD,XPS,microbial sequencing,and metagenomic sequencing,the following conclusions were drawn.The introduction of biochar and iron resulted in the formation of denser and more stable granular sludge.Not only was the enzymatic activity enhanced,but the introduction of iron also led to an increase in the relative abundance of Azospira—A nitrate-dependent ferrous iron-oxidizing bacterium—From 7.71%to 10.81%.This enrichment introduced iron-autotrophic denitrification.The iron-autotrophic denitrification process removed the nitrate byproduct generated by the Anammox reaction.Sufficient nitrite was supplied by this coupled process to support the anammox reaction,the nitrogen removal performance of the Anammox process was maintained under low-temperature stress.关键词
铁/生物炭/低温/厌氧氨氧化/脱氮/废水处理Key words
iron/biochar/low temperature/anaerobic ammonia oxidation/nitrogen removal/wastewater teatment分类
化学化工引用本文复制引用
郭婷,吴朕君,程朗,罗佳宝,席佳乐,崔艳蕾,陈鑫..生物炭和铁添加对低温条件下厌氧氨氧化脱氮的影响[J].化工进展,2026,45(4):2525-2538,14.基金项目
河南省重点研发与推广专项(科技攻关)(242102320087,242102320086) （科技攻关）
郑州市科技局自然科学项目(22ZZRDZX12). （22ZZRDZX12）
