黑龙江八一农垦大学学报2026，Vol.38Issue(2)：25-30,46,7.DOI:10.3969/j.issn.1002-2090.2026.02.004
鹅星状病毒 Spike 蛋白与铁蛋白融合表达及纳米颗粒自组装
Fusion Expression of Goose Astrovirus Spike Protein with Ferritin and Self-Assembly of Nanoparticles
摘要
Abstract
To prepare nanoparticles of goose astrovirus(GoAstV)spike protein,the Spike gene was cloned into the prokaryotic expression vector Fer-pET32a carrying the ferritin gene.After identification of double enzyme digestion and sequencing,the recombinant plasmid was transformed into competent E.coli BL21 cells.The Spike-Fer recombinant protein was induced by IPTG and purified by Ni-column affinity chromatography.The recombinant protein was analyzed and identified by Western blot,transmission electron microscopy,and nanoparticle size analyzer.The results showed that an 880 bp Spike gene fragment was successfully amplified,The constructed Spike-Fer-pET32a recombinant plasmid was digested with restriction endonucleases EcoRⅠ and XhoⅠ,and a vector band at 5 900 bp and a Spike-Fer tandem gene band at 1 425 bp were observed,which was consistent with the expected results.The Spike-Fer recombinant protein was successfully expressed in E.coli mainly in soluble form with a molecular weight of about 70 kDa.The Spike-Fer recombinant protein could specifically react with the anti-His tag monoclonal antibody.Uniform spherical nanoparticles with a diameter of about 50.7 nm formed by self-assembly of the Spike-Fer recombinant protein were observed under transmission electron microscopy.In conclusion,self-assembled spherical nanoparticles of goose astrovirus were successfully prepared,which provides a material basis for the development of a goose astrovirus nanoparticle vaccines.关键词
鹅星状病毒/原核表达/铁蛋白/Spike 蛋白/纳米颗粒Key words
goose astrovirus/prokaryotic expression/ferritin,Spike protein/nanoparticles分类
农业科技引用本文复制引用
杨旭,张美辰,杨丹,朱庆贺,孙东波..鹅星状病毒 Spike 蛋白与铁蛋白融合表达及纳米颗粒自组装[J].黑龙江八一农垦大学学报,2026,38(2):25-30,46,7.基金项目
黑龙江省国家级大学生创新训练计划项目(202510223021) （202510223021）
国家自然科学基金区域创新发展联合基金项目(U23A20236) （U23A20236）
大庆市指导性科技计划项目(zd-2024-29). （zd-2024-29）
