黑龙江八一农垦大学学报2026，Vol.38Issue(2)：54-62,92,10.DOI:10.3969/j.issn.1002-2090.2026.02.008
树莓Ri3GT基因克隆及表达模式分析
Gene Cloning and Expression Pattern Analysis of Ri3GT in Raspberry
摘要
Abstract
To investigate the sequence characteristics,expression pattern,and promoter cis-elements of the raspberry flavonoid 3-O-glucosyltransferase gene(Ri3GT),postharvest raspberry fruits were used as experimental materials.The Ri3GT gene and its promoter sequence were cloned by PCR and subjected to bioinformatic anaylsis,and the expression pattern of Ri3GT was detected by quantitative real-time PCR.The results showed that the full-length cDNA of Ri3GT was 1 395 bp,encoding 464 amino acids,with multiple phosphorylation sites and no signal peptide.The protein belonged to the UDP glucosyltransferase family and contained a PSPG domain.It had the closest genetic relationship with strawberry 3GT,and bound to cyanidin mainly through five types of interactions,including conventional hydrogen bonds and Pi-Cation interactions.The expression level of Ri3GT increased significantly on day 2 of postharvest storage,and this trend was positively correlated with anthocyanin content.Light and methyl jasmonate response elements were identified in the Ri3GT promoter.These results provide a reference for elucidating the role of the Ri3GT gene in anthocyanin biosynthesis in postharvest raspberry fruits.关键词
树莓/花青素/Ri3GT基因/生物信息学分析Key words
raspberry/anthocyanin/Ri3GT gene/bioinformatics analysis分类
农业科技引用本文复制引用
李跃,崔方正,刘盈,钱丽丽,朱思静,孙华军..树莓Ri3GT基因克隆及表达模式分析[J].黑龙江八一农垦大学学报,2026,38(2):54-62,92,10.基金项目
黑龙江省自然科学基金联合引导项目(LH2021C067) （LH2021C067）
黑龙江八一农垦大学引进人才科研启动基金(XYB202211 （）
XYB2021022). （）
