黑龙江农业科学Issue(3)：68-78,11.DOI:10.11942/j.issn1002-2767.2026.03.0068
贵州不同产区杜仲内生菌与根际微生物群落多样性及功能分析
Analysis of Diversity and Function of Bark,Root System and Rhizosphere Microbial Community of Eucommia ulmoides from Different Producing Areas in Guizhou
摘要
Abstract
In order to reveal the intrinsic relationship between endophytic and rhizosphere soil microbial communities in the bark and fibrous roots of E.ucommia ulmoides and their environmental adaptability,High-throughput sequencing was performed on endophytic microorganisms from bark and roots,as well as rhizosphere soil microorganisms of E.ulmoides collected from Daozhen,Weng'an,and Bijie production areas in Guizhou in this study.Bioinformatics methods were employed to analyze the microbial structure,species correlations,and functional predictions.The results showed that,NMDS analysis showed that bacterial communities clustered specifically by tissue compartment(bark,root,soil),while fungal communities were influenced by both production area and tissue compartment.The Bijie production area had the lowest number of endophytic ASVs but the highest number in rhizosphere soil.It also exhibited the highest abundance of potentially pathogenic bacterial phenotypes and the lowest stress tolerance.Dominant bacterial phyla included Pseudomonadota and Actinomycetota,with the abundance of Pseudomonadota in bark and rhizosphere soil showing a trend of DZ＜WA＜BJ,while Actinomycetota showed the opposite pattern.Dominant bacterial genera,such as Sphingomonas,Bradyrhizobium,and Actino phytocola,exhibited tissue specificity.Among fungi,the phylum Glomeromycota reached an abundance of 9.63％in Daozhen roots,and the genus Mortierella was dominant in the rhizosphere soil of Daozhen and Weng'an.Network analysis indicated that microbial corre-lations were tissue-specific,with endophytic and rhizosphere microbes mostly showing negative correlations.Functional prediction revealed differences in microbial phenotypes and trophic types among production areas,with Bijie showing higher potential pathogenicity,and arbuscular mycorrhizal fungi were enriched in Daozhen roots.In conclusion,the microbial community structure of E.ulmoides exhibited clear tissue-specific and pro-duction area-specific characteristics.Community assembly was co-regulated by the micro-environment and pro-duction area conditions.关键词
杜仲/内生菌群/根际菌群/高通量测序/贵州产区Key words
Eucommia ulmoides/endophytic microbiota/rhizospheric microbiota/High-throughput sequencing/Guizhou Production Regions引用本文复制引用
王晨,陈晓芳,任锡毅,龙盼,文阳..贵州不同产区杜仲内生菌与根际微生物群落多样性及功能分析[J].黑龙江农业科学,2026,(3):68-78,11.基金项目
贵州省人才基地项目(RCJD2020-21) （RCJD2020-21）
毕节市联合基金项目(毕科合yz[2021]2号,毕科联合[2025]79号) （毕科合yz[2021]2号,毕科联合[2025]79号）
毕节市科技创新平台及人才团队(毕科合[2023]66号) （毕科合[2023]66号）
毕节医学高等专科学校科研团队项目(BJYZXT202401). （BJYZXT202401）
