华南农业大学学报2026，Vol.47Issue(3)：442-452,11.DOI:10.7671/j.issn.1001-411X.202507034
广东黄羽肉种鸡传染性贫血病毒的流行病学调查与分析
Epidemiological investigation and analysis of infectious anemia virus in yellow-feathered broiler breeder chickens of Guangdong Province
摘要
Abstract
[Objective]To investigate the epidemiological characteristics of chicken infectious anemia virus(CIAV)in yellow-feathered broiler breeders in Guangdong,thereby providing a scientific basis for regional CIAV prevention and control strategies.[Method]From March 2024 to January 2025,a total of 787 serum samples and 180 tissue samples(liver and spleen)were collected from six large-scale,unvaccinated yellow-feathered broiler breeder farms in Guangdong.Serum samples were obtained from parent flocks,while tissue samples were collected from deceased chicks under three weeks of age.Serum antibody detection,virus isolation,and whole-genome sequencing were then employed to investigate the prevalence and molecular characteristics of CIAV in these flocks.[Result]Serum antibody ELISA result revealed varying degrees of CIAV infection across all six farms,with an overall antibody-positive rate of 50.8%(400/787).Farm-level positive rates ranged from 32.0%to 77.8%,and increased with age,rising from 27.0%in flocks younger than 7 weeks to 89.6%in those aged 46-57 weeks.PCR testing of tissue samples from diseased chicks yielded a positive rate of 55.0%(99/180).Using MDCC-MSB1 cells,11 CIAV strains were successfully isolated from the positive diseased samples.Whole-genome sequencing revealed that all 11 CIAV isolates had a genome length of 2 298 bp,with no base deletion or insertion.Nucleotide sequence similarity among the isolates ranged from 97.6%to 99.9%.Genetic evolution analysis classified 10 isolates as genotype IIIa and one isolate(GD24QY8)as genotype IIIb.All isolates possessed the glutamine(394Q)amino acid residue,a molecular characteristic associated with highly pathogenic CIAV strains.[Conclusion]CIAV infection is widespread in Guangdong's yellow-feathered broiler breeder population,with the predominant strains being genotype IIIa strains that possesses molecular characteristic of high pathogenicity strains.These findings underscore the need for enhanced surveillance and implementation of effective control measures against CIAV.关键词
鸡传染性贫血病毒/黄羽肉种鸡/流行病学调查/病毒分离与鉴定/全基因组序列分析Key words
Chicken infectious anemia virus/Yellow-feathered broiler breeder chicken/Epidemiological investigation/Virus isolation and identification/Whole-genome sequence analysis分类
农业科技引用本文复制引用
舒雪利,曹伟胜,周婉怡,陈钦玺,王红梅,张金玉,王伟彬,樊星,苏桐,廖明..广东黄羽肉种鸡传染性贫血病毒的流行病学调查与分析[J].华南农业大学学报,2026,47(3):442-452,11.基金项目
广东省现代农业产业技术体系(2023KJ128,2024CXTD20) （2023KJ128,2024CXTD20）
国家现代农业产业技术体系(CARS-41) （CARS-41）
