华南农业大学学报2026，Vol.47Issue(3)：465-473,9.DOI:10.7671/j.issn.1001-411X.202507017
金色轮生链霉菌GD7-17的分离鉴定及对香蕉枯萎病的生防潜力
Isolation and identification of Streptomyces aureoversilis GD7-17 and its biocontrol potential against banana Fusarium wilt
摘要
Abstract
[Objective]To screen and identify effective biocontrol agent against banana Fusarium wilt.[Method]Antagonistic bacteria against Fusarium oxysporum f.sp.cubense tropical race 4(Foc TR4)were screened from the rhizosphere soil of healthy banana plants by plates confrontation method.The antagonistic strain was identified by morphological observation,physiological and biochemical tests,and phylogenetic analysis of multiple genes including 16S rDNA,GyrB,RpoB and TrpB.The effect of the antagonistic strain on Foc TR4 hyphae morphology and conidial germination was evaluated using microscopic examination,and its antifungal spectrum was investigated by the plate confrontation method.Volatile organic compounds(VOCs)from the antagonistic strain were analyzed through gas chromatography-mass spectrometry(GC-MS).The biological control effect and plant growth-promoting effect were evaluated by pot experiments under greenhouse conditions.[Result]A strain,designated GD7-17,was isolated and identified as Streptomyces aureoversilis.It exhibited strong antagonistic activity against Foc TR4,inhibiting mycelial growth by 72.19%and inducing abnormal hyphal morphology.Furthermore,the strain showed broad-spectrum antifungal activity against plant pathogenic fungi.VOCs produced by strain GD7-17 significantly inhibited fungal growth,with an inhibition rate of 76.9%.Six VOCs were identified through GC-MS,including geosmin and 2-methylisoborneol.Furthermore,S.aureoversilis GD7-17 exhibited a biocontrol efficacy of 58.2%against banana Fusarium wilt disease,and simultaneously exerted a significant growth-promoting effect on banana plants,resulting in an 18.0%increase in fresh weight,an 8.2%increase in corn diameter,and a 16.7%increase in plant height.Strain GD7-17 was able to degrade lignin,secrete proteases and solubilize inorganic phosphate.[Conclusion]S.aureoversilis GD7-17 exhibits promising biocontrol potential against banana Fusarium wilt.This study provides a potential microbial agent for biocontrol of banana Fusarium wilt.关键词
香蕉枯萎病/尖孢镰刀菌古巴专化型/金色轮生链霉菌/生物防治/促生作用Key words
Banana Fusarium wilt/Fusarium oxysporum f.sp.cubense/Streptomyces aureoversilis/Biological control/Growth-promoting effect分类
农业科技引用本文复制引用
蔡雨霏,陆月媚,曾莉莎,朱杰,郑利乐,李华平,李云锋,聂燕芳..金色轮生链霉菌GD7-17的分离鉴定及对香蕉枯萎病的生防潜力[J].华南农业大学学报,2026,47(3):465-473,9.基金项目
国家现代农业产业技术体系建设专项(CARS-31) （CARS-31）
广东省基础与应用基础研究基金(2022A1515140114) （2022A1515140114）
广东省现代农业产业技术体系创新团队建设项目(2024CXTD21) （2024CXTD21）
国家自然科学基金(31600663) （31600663）
