农业科技与技术（英文）2025，Vol.26Issue(4)：39-45,80,8.DOI:10.16175/j.cnki.1009-4229.2025.04.005
芥菜型油菜BjB01.MTP5启动子的表达载体构建及表达特性分析
Promoter Expression Vector Construction and Expression Profiling of BjB01.MTP5 in Brassica juncea
摘要
Abstract
In this study,we successfully constructed the promoter expression vector ProBjB01.MTP5-GUS for BjB01.MTP5 in Brassica juncea.Phylogenetic and cis-acting element analyses were performed based on the promoter sequences of the MTP5 gene from ten Brassicaceae species.The results revealed that the MTP5 promoters of the B subgenome in Brassica formed distinct evolutionary branches compared with those of the A/C subgenomes.Analysis of cis-acting elements indicated that elements responsive to phytohormones(e.g.,abscisic acid,methyl jasmonate,and auxin)and abiotic stresses including anaerobic induction,salicylic acid,drought,and low temperatures were highly conserved in the MTP5 promoters across different species.Tissue-specific expression profiling demonstrated that BjB01.MTP5 was predominantly expressed in roots,flower buds,siliques,and pericarps.Furthermore,qRT-PCR analysis under heavy metal stress showed that the expression of BjB01.MTP5 was significantly induced by various heavy metals,such as Mn2+,Fe2+,Zn2+,and Sb3+.Its expression pattern correlated strongly with the distribution of heavy metal-responsive elements in the promoter region.These findings provided important theoretical insights into the biological functions of MTP genes in response to heavy metal stress.关键词
芥菜型油菜/BjB01.MTP5/启动子分析/重金属应答Key words
Brassica juncea/BjB01.MTP5/Promoter analysis/Heavy metal response引用本文复制引用
盛佳林,龚莎,钟品田,陈明哲,袁玉辉,游亮..芥菜型油菜BjB01.MTP5启动子的表达载体构建及表达特性分析[J].农业科技与技术（英文）,2025,26(4):39-45,80,8.基金项目
Supported by Undergraduates'Innovation and Entrepreneurship Training Program(S202410553006) （S202410553006）
Natural Science Foundation of the Department of Science and Technology of Hunan Province(2025JJ70318) （2025JJ70318）
Excellent Youth Project of the Education Department of Hunan Province(23B0809). 大学生创新创业训练计划项目(S202410553006) （23B0809）
湖南省科技厅自然科学基金项目(2025JJ70318) （2025JJ70318）
湖南省教育厅优秀青年项目(23B0809) （23B0809）
