农业科技与技术（英文）2025，Vol.26Issue(4)：66-80,15.DOI:10.16175/j.cnki.1009-4229.2025.04.008
阿魏酸对H2O2诱导的猪肠道上皮细胞氧化损伤的保护作用
Ferulic Acid Protects Porcine Intestinal Epithelial Cells Against H2O2-Induced Oxidative Damage
摘要
Abstract
To elucidate the underlying mechanisms of ferulic acid(FA)protects against hydrogen peroxide(H2O2)-induced oxidative damage,this study compared the differences in the types and content of phenolic acids such as ferulic acid(FA)in the whole and peeled fruit juice samples of Gannan navel oranges.Using the H2O2 treatment group as the control,the effect of FA treatment on porcine intestinal epithelial cells(IPEC-J2)were analyzed,including:superoxide dismutase(SOD)activity,catalase(CAT)activity,malondialdehyde(MDA)content,reactive oxygen species(ROS)levels,cell morphology,cell viability,lactate dehydrogenase(LDH)leakage rate,and mitochondrial membrane potential.The results revealed that the content of FA in the whole fruit juice was 5.8 times of that in the peeled fruit juice.The results showed that the content of FA in the whole fruit juice was 5.8 times that in the peeled fruit juice.FA markedly ameliorated H2O2-induced cell vitality loss and apoptosis in IPEC-J2.The protective efficacy of FA might be ascribed to the increases in superoxide dismutase(SOD)and catalase(CAT)activities and the decreases in lactate dehydrogenase(LDH)release,reactive oxygen species(ROS),and malondialdehyde(MDA)in IPEC-J2 cells.Meanwhile,our study revealed correlations between mitochondrial Cyt-c-mediated caspase-3 activation pathway and the antioxidant effect of FA on IPEC-J2.Furthermore,FA reduced the activation of p53 and caspase-8 mediated by H2O2.Taken together,our results indicated that FA protected IPEC-J2 cells against H2O2-mediated oxidative stress and cell injury by resistance to apoptosis via the inhibition of p53-and caspase-3-dependent signaling pathways.The current study indicated that FA could serve as a potentially effective candidate medicine for the treatment of intestinal oxidative damage.关键词
阿魏酸/过氧化氢/肠上皮细胞/氧化损伤Key words
Ferulic acid/Hydrogen peroxide/Intestinal epithelial cells/Oxidative damage引用本文复制引用
张蔚清,张宇桂,黄绿红,窦艳华,梁曾恩妮..阿魏酸对H2O2诱导的猪肠道上皮细胞氧化损伤的保护作用[J].农业科技与技术（英文）,2025,26(4):66-80,15.基金项目
Supported by Natural Science Foundation of Changsha City(kq2208104) （kq2208104）
Agricultural Science and Technology Innovation Fund Project of Hunan Province(2025CX110) （2025CX110）
National Natural Science Foundation of China(U25A20730) （U25A20730）
General Projects of Dongting Laboratory(2025-DTYB-002) 长沙市自然科学基金(kq2208104) （2025-DTYB-002）
湖南省农业科技创新资金项目(2025CX110) （2025CX110）
国家自然科学基金项目(U25A20730) （U25A20730）
洞庭实验室一般项目(2025-DTYB-002) （2025-DTYB-002）
