华侨大学学报（自然科学版）2026，Vol.47Issue(3)：309-317,9.DOI:10.11830/ISSN.1000-5013.202505066
粘质沙雷氏菌突变几丁质酶C的包涵体复性
Renaturation of Inclusion Bodies of Serratia marcescens Mutant Chitinase C
摘要
Abstract
To screen the composition of the renaturation buffer for inclusion bodies of the Serratia marcescens mutant chitinase C expressed in E.coli BL21(DE3),the inclusion bodies were isolated,washed,and purified by metal chelate affinity chromatography.Using fractional factorial design,13 factors were examined,and en-zyme activity and protein solubility were set as response indicators to optimize the refolding solution of chit-i nase C inclusion bodies and evaluate the refolding effect.The results showed that the optimal refolding solution composition was 2 mmol·L-1 divalent cations,urea,5 mmol·L-1β-mercaptoethanol,NaCl and KCl concen-tration ratio of 250 mmol·L-1∶10 mmol·L-1,0.1%mass fraction of PEG 6000,0.5 mol·L-1 sorbitol,and pH 8.5.Under these conditions,the refolding yield of chitinase C inclusion bodies reached 77.9%.关键词
几丁质酶C/包涵体/复性/分式析因设计Key words
chitinase C/inclusion bodies/renaturation/fractional factorial design分类
生物科学引用本文复制引用
刘嘉,贺淹才..粘质沙雷氏菌突变几丁质酶C的包涵体复性[J].华侨大学学报（自然科学版）,2026,47(3):309-317,9.基金项目
华侨大学实验教学与管理改革项目(612-50423031) （612-50423031）
