湖南农业大学学报（自然科学版）2026，Vol.52Issue(1)：11-17,7.DOI:10.13331/j.cnki.jhau.2026.01.002
甘蓝型温敏雄性不育油菜W21的育性转换及花药发育的细胞学研究
Fertility transition and cytological characteristics of anther development in thermo-sensitive genic male sterile rapeseed line W21
摘要
Abstract
Using the thermo-sensitive genic male sterile(TGMS)Brassica napus line W21 as the experimental material,this study systematically investigated the critical period,characteristics,and inheritance pattern of pollen abortion by combining morphological observation,genetic analysis,and cytological examination.The results showed that the critical fertility transition temperature of W21 was 22℃.Under 18℃,the plants exhibited complete male fertility,with normal stamen development,bright yellow anthers,and plump pollen grains.In contrast,when the temperature exceeded 22℃,anther degeneration occurred,leading to sterility.The sterility trait of W21 was controlled by at least two pairs of recessive nuclear genes.The pollen abortion process of W21 initiated at the pollen mother cell stage and culminated in complete abortion during the middle-late uninucleate stage.The key cytological characteristics of abortion include:abnormal development of the anther epidermal structure;premature degeneration of the tapetum accompanied by abnormal vacuolization;formation of abnormal tetrads due to meiotic irregularities in pollen mother cells;and defects in pollen exine development.These abnormalities ultimately resulted in microspore adhesion and cytoplasmic degradation,leading to complete pollen abortion.关键词
甘蓝型油菜/温敏雄性不育/遗传特性/细胞学Key words
Brassica napus/thermo-sensitive genic male sterile/genetic characteristics/cytology分类
农业科技引用本文复制引用
熊佳仁,刘艳欣,王艳红,张振乾,肖钢..甘蓝型温敏雄性不育油菜W21的育性转换及花药发育的细胞学研究[J].湖南农业大学学报（自然科学版）,2026,52(1):11-17,7.基金项目
科技创新2030—重大项目(2022ZD04010) （2022ZD04010）
