湖南农业大学学报（自然科学版）2026，Vol.52Issue(1)：18-24,7.DOI:10.13331/j.cnki.jhau.2026.01.003
玉米ZmMDR1基因的抗病功能解析
Functional analysis of disease resistance gene ZmMDR1 in maize
摘要
Abstract
Using qRT-PCR,luminol-based chemiluminescence assays,and maize protoplast subcellular localization,we evaluated the resistance of the Zmmdr1 mutant to southern corn rust and corn leaf blight and analyzed its immune responses.The results showed that in the Zmmdr1 mutant,the nucleotide at position 1997 bp of the ZmMDR1 gene was changed from G to A,resulting in premature termination of translation.After inoculation with the pathogens causing southern corn rust and corn leaf blight,the leaves of Zmmdr1 mutants showed significantly more spores and lesions compared to wild-type plants,indicating a marked reduction in resistance to both diseases in Zmmdr1 mutants.ROS levels following treatment with Flg22 were detected,and ROS production in Zmmdr1 mutants was found to be markedly lower than that in the wild-type.After pathogen inoculation,the expression of the key immune-related genes ZmrbohD and ZmPR1 was analyzed,and the expression of the two immune marker genes was found to be significantly reduced in Zmmdr1 mutants compared to the wild-type,suggesting that ZmMDR1 plays an essential role in the immune response.Subcellular localization using a maize protoplast system revealed that ZmMDR1 is a plasma membrane-localized protein.关键词
玉米/玉米南方锈病/玉米小斑病/抗病基因/ZmMDR1Key words
maize(Zea mays L.)/southern corn rust/corn leaf blight/resistance gene/ZmMDR1分类
农业科技引用本文复制引用
胥启东,孙娟,贾富成,耿闯,杨佳乐,吕蒙,李新薇,李金辉,牛洪斌,郑天慧..玉米ZmMDR1基因的抗病功能解析[J].湖南农业大学学报（自然科学版）,2026,52(1):18-24,7.基金项目
河南省优秀青年科学基金项目(232300421036) （232300421036）
国家自然科学基金面上项目(32272175) （32272175）
