湖南农业大学学报（自然科学版）2026，Vol.52Issue(1)：25-32,8.DOI:10.13331/j.cnki.jhau.2026.01.004
长沙烟草赤星病致病菌的生物学特性及其防治药剂筛选
Biological characteristics of the pathogen causing tobacco brown spot in Changsha and screening of its control agents
摘要
Abstract
Brown spot disease is one of the main foliar diseases endangering the safe production of tobacco leaves in the Changsha tobacco-growing area.Previous studies have shown that Alternaria tenuissima(A.tenuissima)and Alternaria alternata(A.alternata)are the pathogenic fungi of brown spot disease in the Changsha tobacco-growing area.To clarify the biological characteristics of the two pathogens,this study compared the effects of temperature,light,carbon sources,and nitrogen sources on mycelial growth and sporulation.Furthermore,the indoor toxicity of nine chemical agents against the pathogens was investigated,and highly effective agents were selected for field trials.The biological charac-teristics experiments revealed that the optimal growth temperatures for A.tenuissima and A.alternata were 25 ℃ and 30 ℃,respectively.The optimal carbon and nitrogen sources for A.tenuissima were glucose and sodium nitrate,and dark culture promoted sporulation.For A.alternata,the optimal carbon and nitrogen sources were maltose and peptone,and a 12 h dark/12 h light cycle favored sporulation.Indoor toxicity tests indicated significant differences in the toxicity of the nine fungicides against the two Alternaria species.For A.tenuissima,fluazinam exhibited the strongest toxicity(EC50=1.326 2 mg/L),while thifluzamide was the weakest(EC₅₀=5 121.437 3 mg/L).For A.alternata,fluazinam showed the highest toxicity(EC₅₀=0.004 8 mg/L),and picoxystrobin was the least effective(EC₅₀=8 052.050 0 mg/L).Field trial results demonstrat-ed that 40%dimetachlone achieved a control efficacy of 67.70%,followed by 500 g/L fluazinam(66.66%),32.5%benzo-yl-azoxystrobin(54.86%),and 50%boscalid(25.50%).Based on the indoor toxicity and field trial results,alternating ap-plications of 40%dimetachlone,500 g/L fluazinam,and 32.5%benzoyl-azoxystrobin are recommended for chemical control of brown spot disease in the Changsha tobacco-growing area.关键词
烟草赤星病/细极链格孢/链格孢/生物学特性/室内毒力测定/防效/长沙烟区Key words
tobacco brown spot disease/A.tenuissima/A.alternata/biological characteristics/indoor toxicity assay/con-trol efficacy/Changsha tobacco-growing area分类
农业科技引用本文复制引用
蔡海林,陈武,谢林,邢云飞,徐灵超,李开来,李建勇,何昊欣,黄彬彬,刘清术..长沙烟草赤星病致病菌的生物学特性及其防治药剂筛选[J].湖南农业大学学报（自然科学版）,2026,52(1):25-32,8.基金项目
湖南省烟草公司长沙市公司科技项目(CS2022KJ02) （CS2022KJ02）
中国烟草总公司湖南省公司科技项目(HN2024KJ04) （HN2024KJ04）
湖南中烟工业责任有限公司科技项目(KY2025JD0004) （KY2025JD0004）
