不同温度、盐度下日本虎斑猛水蚤(Tigriopus japonicus)对四种海洋微藻的摄食行为研究
Optimal feeding behavior of Tigriopus japonicus with four marine microalgae at different carbon contents,temperatures,and salinity levels
摘要
Abstract
Tigriopus japonicus is a high-quality live feed organism with great potential for application in aquaculture.How-ever,T.japonicus is sensitive to culture conditions,and large-scale cultivation remains challenging resulting in limited pro-duction that cannot meet the needs of intensive aquaculture.Therefore,an accurate control of culture conditions is essential to maintain its growth and reproduction.To optimize the cultivation conditions,the feeding behavior of T.japonicus regard-ing four marine microalgae—Platymonas helgolandica,Phaeodactylum tricornutum,Isochrysis galbana,and Chlorella vulgaris—was observed under varied conditions.These include five carbon contents:0.5,1.0,1.5,2.5,and 5 μg C/mL;un-der four temperatures:15℃,20℃,25℃,and 30℃;and four salinity levels:15,25,30,and 35 psu.The grazing,clearance,and ingestion rates of T.japonicus concerning the four microalgae peaked at 25℃;the suitable temperature range was 25℃-30℃.The feeding ability was generally optimal at salinity 30.The feeding behavior was the best in the range of 2.5-5.0 μgC/mL,with a peak at 2.5 μgC/mL.The feeding preference was P.helgolandica>I.galbana>C.vulgaris>P.tricornutum,and the optimal equivalent spherical diameter of the feed was～21.3 μm.Thus,the feeding behavior of T.ja-ponicus with four marine microalgae was the best at 25℃,30 salinity,and 2.5 μgC/mL carbon.P.helgolandica was selected as the ideal feed,which provided an experimental basis for the artificial culture of T.japonicus.关键词
日本虎斑猛水蚤(Tigriopus japonicus)/海洋微藻/摄食行为/摄食碳占体碳比值/温度/盐度Key words
Tigriopus japonicus/marine microalgae/feeding behavior/the ratio of carbon intake to body carbon/temperature/salinity分类
农业科技引用本文复制引用
刘子瑜,栾佳颐,马爱军,闫鹏飞,商晓梅,孙志宾,高云衣,赵艳飞,王新安..不同温度、盐度下日本虎斑猛水蚤(Tigriopus japonicus)对四种海洋微藻的摄食行为研究[J].海洋科学,2025,49(10):64-78,15.基金项目
国家重点研发项目(2022YFE0203900) （2022YFE0203900）
广东省现代化海洋牧场适养品种核心技术攻关项目(NJTG-20250402) （NJTG-20250402）
国家自然科学基金项目(42206087) （42206087）
天津市海洋资源与化学重点实验室开放基金资助项目(202301)国家重点研发计划(2022YFD2400403) （202301）
现代农业产业技术体系(CARS-47-G01) （CARS-47-G01）
中国水产科学研究院中央级公益性科研院所基本科研业务费(2023TD26) National Key Research and Development Project,No.2022YFE0203900 （2023TD26）
Guangdong Provincial Core Technology Research Project for Suitable Breeds in Modern Marine Ranches,No.NJTG-20250402 （）
National Natural Science Foundation of China,No.42206087 （）
Tianjin Key Laboratory of Marine Resources and Chemistry Open Fund,No.202301 （）
National Key Research and Development Program,No.2022YFD2400403 （）
Modern Agricul-tural Industry Technology System,No.CARS-47-G01 （）
Basic Scientific Re-search Fund for Central Public Welfare Research Institutes of Chinese Academy of Fishery Sciences,No.2023TD26 （）
