海洋科学2025，Vol.49Issue(10)：99-110,12.DOI:10.11759/hykx20251009001
酶解法制备牡蛎肽的工艺优化及抗氧化作用研究
Optimization of enzyme-based preparation conditions for oyster peptides and evaluation of their antioxidant activity
摘要
Abstract
To enhance the comprehensive utilization of oyster protein and optimize enzymatic hydrolysis process for oyster peptide production,trypsin,Protamex®,and Flavourzyme® were used to hydrolyze oyster protein.Hy-droxyl radical scavenging activity was employed as the evaluation index to assess the effects of hydrolysis time,enzyme dosage,liquid-to-solid ratio,temperature,and pH on hydrolysis efficiency.Based on single-factor experi-ments,an orthogonal experimental design was used to optimize processing parameters.Subsequently,orthogonal dual-enzyme hydrolysis experiments were conducted to prepare oyster peptides under different hydrolysis condi-tions.The results showed that oyster peptides with the best performance were obtained using trypsin and Prota-mex® at a total enzyme dosage of 2000 U/g(trypsin:Protamex®=1:3),pH 7.0,hydrolysis time of 6.5 h,a liq-uid-to-solid ratio of 2:1,and a hydrolysis temperature of 50℃.Under these conditions,the soluble peptide content reached 50.43％,the degree of hydrolysis was 48.62％,and the hydroxyl radical scavenging activity was as high as 75.49％.Low-molecular-weight peptides(≤1 000 Da)accounted for 50％-70％of the total peptides.Moreover,the oyster peptides were rich in hydrophobic amino acids,which likely contributed to their strong antioxidant activity.This study provides a scientific basis for the targeted preparation of oyster peptides and supports the development of marine functional foods.关键词
牡蛎肽/酶解工艺/正交实验/双酶复合水解/抗氧化活性Key words
oyster peptide/enzymatic hydrolysis process/orthogonal test/dual-enzyme complex hydrolysis/antioxidant activity分类
轻工纺织引用本文复制引用
李帅欣,王兴仁,王雪芹,宋琳..酶解法制备牡蛎肽的工艺优化及抗氧化作用研究[J].海洋科学,2025,49(10):99-110,12.基金项目
山东省自然科学基金(ZR2023MD095) （ZR2023MD095）
现代农业产业技术体系专项(CARS-49) Natural Science Foundation of Shandong Province,No.ZR2023MD095 （CARS-49）
the Earmarked Fund for Modern Agro-industry Technology Research System,No.CARS-49 （）
