海洋科学2025，Vol.49Issue(11)：28-37,10.DOI:10.11759/hykx20250925001
香港牡蛎及其养殖水体中食源性致病菌的周年变动规律及环境相关性
Annual variations and environmental correlations of food-borne bacterial load in Hong Kong oysters(Crassostrea hongkongensis)and their aquaculture waters
摘要
Abstract
To clarify annual variations in the loads of foodborne bacteria and their influence on oyster(Crassostrea hongkongensis)cultivation in the Hainan province,this study quantified the total bacterial counts and abundances in various tissue types of five oyster species and aquaculture water using plate counting combined with polymerase chain reaction(PCR).The plausible correlations between bacterial infection levels and environmental factors were also analyzed.The results showed that:(1)the species Escherichia coli,Vibrio parahaemolyticus,V.vulnificus,and Listeria monocytogenes dominated,with maximum concentrations of 6.3×10⁴,2.9×10⁴,6.1×10⁴,and 1.4×10⁵CFU/g,respectively.The levels were significantly higher in all oyster tissues than in aquaculture water.Salmonella was detected only in specific tissues during certain months:2.3×10³ CFU/g in the gills and 2.1×10⁴ CFU/g in the digestive glands in July and September;(2)tissue and aquaculture water levels of each bacterium and the total bac-terial count exhibited clear and consistent seasonal fluctuations,with abundance peaks detected in February-March and August-October;(3)maximal bacterial loads were found in the gills and mantle,followed by the digestive gland,adductor muscle,and gonads;(4)environmental factors and bacterial concentrations were weakly correlated.Bacterial levels in aquaculture water were positively correlated with salinity and pH,but negatively correlated with temperature and chlorophyll-a concentrations.In contrast,E.coli,V.vulnificus,and V.parahaemolyticus contents in the gills and mantle were directly proportional to temperature and chlorophyll-a concentrations.This study pro-vides a scientific basis for understanding the annual dynamics of foodborne bacterial infections in oysters and serves as an early warning system for related food safety risks.关键词
香港牡蛎(Crassostrea hongkongensis)/食源性致病菌/环境因子/相关性/周年变化Key words
Crassostrea hongkongensis/foodborne bacteria/environmental factors/correlation/annual variation分类
农业科技引用本文复制引用
胡青松,杨文红,鲍凌翔,洪鑫,孙灏,周杜源,刘春胜..香港牡蛎及其养殖水体中食源性致病菌的周年变动规律及环境相关性[J].海洋科学,2025,49(11):28-37,10.基金项目
海南省重点研发计划项目(ZDYF2023XDNY032,ZDYF2024SHFZ139) （ZDYF2023XDNY032,ZDYF2024SHFZ139）
三亚科技"繁星"专项项目(2024KJFX019) Key Research and Development Project of Hainan Province,Nos.ZDYF2023XDNY032,ZDYF2024SHFZ139 （2024KJFX019）
Sanya Science and Technology Star Project,No.2024KJFX019 （）
