海洋科学2025，Vol.49Issue(12)：81-91,11.DOI:10.11759/hykx20250725001
催化报告沉积-荧光原位杂交CARD-FISH技术在有害藻华研究中的应用前景及与主流分子检测技术的比较
Application prospects of catalyzed reporter deposi-tion-fluorescence in situ hybridization in the research of harmful algal blooms and comparison with mainstream mo-lecular detection techniques
摘要
Abstract
Environmental changes and human activities have intensified the frequent occurrence of harmful algal blooms(HABs),posing serious threats to ecosystems and human health.Therefore,it is crucial to develop efficient and sensitive detection technologies for accurately monitoring the occurrence and development of HAB species and for precisely monitoring and managing microalgal communities.This article reviews the cutting-edge applications of catalyzed reporter deposition-fluorescence in situ hybridization(CARD-FISH)in the quantitative study of HABs and provides a comparative analysis with three other mainstream techniques:high-throughput sequencing technolo-gies,quantitative real-time polymerase chain reaction,and fluorescence in situ hybridization.In addition,this paper highlights that CARD-FISH possesses unique advantages,such as high sensitivity and in situ quantification,making it highly valuable in the quantitative study of HABs.Furthermore,the article discusses the technique's application prospects and future development potential,providing theoretical support and technical reference for related re-search.关键词
赤潮藻/分子鉴定/CARD-FISHKey words
harmful algal blooms/molecular identification/CARD-FISH分类
生物科学引用本文复制引用
郑丽华,韩笑天,徐秀丽..催化报告沉积-荧光原位杂交CARD-FISH技术在有害藻华研究中的应用前景及与主流分子检测技术的比较[J].海洋科学,2025,49(12):81-91,11.基金项目
山东省自然科学基金面上项目(ZR2022MD031)General Program of Shandong Provincial Natural Science Foundation,No.ZR2022MD031 （ZR2022MD031）
