海洋科学2025，Vol.49Issue(12)：104-114,11.DOI:10.11759/hykx20250430002
固定剂和保存条件对桡足类及其肠道内容物DNA质量的影响
Effects of the fixative and storage duration on the quality of DNA obtained from copepods and their gut contents
摘要
Abstract
The DNA barcoding technique has been widely used in studying the diversity of copepods and their diet in situ,serving as a powerful tool for understanding the plankton community structure and marine food web dy-namics.Effective sample preservation is a critical prerequisite for downstream DNA analyses,including PCR am-plification and gene sequencing.In this study,we evaluated the effects of the fixative and storage duration on both the yield and quality of DNA extracted from copepods and their gut contents.DNA integrity was assessed through the qPCR quantification of 18S rDNA fragments with different lengths.Our results showed that RNAlater exhibited optimal performance in fixing and preserving copepod DNA with the highest yield and integrity over a two-month storage period.Ethanol demonstrated the best efficacy in the fixation and preservation of gut content DNA as it exhibits excellent permeability and rapidly terminates the physiological and metabolic activities of copepods and gut microbiota,respectively.Neutral Lugol's solution can achieve immediate or short-term fixation of gut content DNA;however,it is unsuitable for long-term preservation.These findings provide insights into sampling strategies,fixation protocols,and storage conditions for investigating copepods and their diet in situ.They guide the use of appropriate fixatives and preservation conditions based on research priorities(copepod DNA or gut content DNA).关键词
桡足类/肠道内容物/固定剂/保存条件/DNAKey words
copepod/gut content/fixation/storage/DNA分类
海洋科学引用本文复制引用
王建龙,侯天琪,王欣欣,庄昀筠,陈洪举,刘光兴..固定剂和保存条件对桡足类及其肠道内容物DNA质量的影响[J].海洋科学,2025,49(12):104-114,11.基金项目
国家自然科学基金项目(41876156)National Natural Science Foundation of China,No.41876156 （41876156）
