家畜生态学报2026，Vol.47Issue(4)：56-61,6.DOI:10.3969/j.issn.1673-1182.2026.04.008
低聚木糖对奶牛瘤胃体外发酵、甲烷产量和微生物菌群的影响
Effects of Xylo-oligosaccharides on In Vitro Rumen Fermentation,Methane Production and Microbial Flora of Dairy Cows
摘要
Abstract
This study was conducted to investigate the effects of xylooligosaccharides(XOS)on rumen fermentation,methane(CH4)production,and microbial populations in vitro.Rumen fluid was collected from three Holstein cows in mid-lactation.Using 2 g of total mixed ration(TMR)as the fermentation sub-strate,XOS were added at concentrations of 0％(control group),0.1％,0.2％,0.4％,and 0.8％.After 24 h of in vitro fermentation,gas production and CH4 production were measured.Fermentation fluid and substrate resi-dues were collected to determine volatile fatty acids(VFA)and in vitro dry matter digestibility(IVDMD).Real-time quantitative PCR was used to quantify microbial populations in the fermentation fluid from the control group and the 0.4％XOS group.The results showed that:(1)Compared with the control group,gas production in all experimental groups was significantly reduced(P＜0.05),and CH4 production in the 0.4％XOS group was signif-icantly decreased(P＜0.05).(2)The IVDMD in the 0.4％XOS group was significantly higher than that in the control,0.1％XOS,and 0.2％XOS groups(P＜0.05).There were no significant differences in acetate,propio-nate,and butyrate concentrations among all groups(P＞0.05).The acetate-to-propionate ratio in the 0.1％XOS and 0.4％XOS groups was significantly lower than that in the other three groups(P＜0.05),but no significant difference was observed between the 0.1％XOS and 0.4％XOS groups(P＞0.05).(3)Com-pared with the control group,the populations of total bacteria,protozoa,methanogens,and Ruminococcus flavefaciens in the 0.4％XOS group were significantly reduced(P＜0.05),while there were no signifi-cant differences in the populations of Fibrobacter succinogenes and Ruminococcus albus between the two groups(P＞0.05).(4)Significant correlations were observed between changes in microbial populations and fermentation parameters as well as CH4 production:the reductions in methanogen and protozoa popu-lations were directly associated with decreased CH4 production,and the optimized acetate-to-propionate ra-tio further inhibited CH4 generation.Therefore,supplementation with 0.4％XOS can reduce CH4 produc-tion by decreasing the abundances of methanogens and protozoa and optimizing the acetate-to-propionate ratio.These findings provide a novel strategy for mitigating CH4 emissions in ruminants.关键词
低聚木糖/奶牛/体外/瘤胃发酵/甲烷/菌群Key words
xylo-oligosaccharide/dairy cows/in vitro/rumen fermentation/methane/microbial flora分类
农业科技引用本文复制引用
韩佳佳,李晗,孔冰月,张琳婕,高腾云,付彤,苏传友..低聚木糖对奶牛瘤胃体外发酵、甲烷产量和微生物菌群的影响[J].家畜生态学报,2026,47(4):56-61,6.基金项目
河南省科技攻关项目(242102110064) （242102110064）
河南省大学生创新训练计划项目(202410466080) （202410466080）
