经济动物学报2026，Vol.30Issue(2)：112-117,6.DOI:10.13326/j.jea.2026.2097
不同方法对貉毛囊基因组DNA提取效果比较
Comparison of Genomic DNA Extraction Effects from Hair Follicles of Raccoon Dog Using Different Methods
摘要
Abstract
Genomic DNA is one of the essential materials for molecular breeding,and using hair follicles to extract genomic DNA is a simple and efficient method.To compare the effects of different extraction methods,the number of raccoon dog hair follicles and sampling sites on the efficiency of genomic DNA extraction,with the aim of determining the optimal protocol for extracting genomic DNA from raccoon dog hair follicles,40 healthy raccoon dogs of different ages and genders were selected.Hair follicle samples were collected from the mid-dorsal region and tail of each individual.DNA was extracted using the centrifugal column method and the magnetic bead method,and the efficiency of genomic DNA extraction was compared based on the number of hair follicles and sampling sites.The results showed that the centrifugal column method outperformed the magnetic bead method,and extracting 100 or more hair follicles could meet the requirements for subsequent experiments.Additionally,the sampling site had no significant impact on DNA extraction quality.The findings provide a scientific approach for obtaining genomic materials from fur-bearing animals such as raccoon dog and lay a foundation for genetic breeding research.关键词
貉/毛囊/DNA提取/部位Key words
raccoon dog/hair follicle/DNA extraction/locations分类
农业科技引用本文复制引用
李佳乐,王明烁,胡梦娇,王浩龙,孙伟丽,赵海平,刘振,周李生..不同方法对貉毛囊基因组DNA提取效果比较[J].经济动物学报,2026,30(2):112-117,6.基金项目
山东省特种经济动物产业技术体系项目(SDAIT-21-22),青岛农业大学高层次人才引进项目(1121009) （SDAIT-21-22）
