吉林农业大学学报2026，Vol.48Issue(2)：212-220,9.DOI:10.13327/j.jjlau.2021.1657
玉米ZmTCP14基因的筛选、生物信息学分析及植物表达载体的构建
Screening,Bioinformatics Analysis and Construction of Plant Expres-sion Vector of ZmTCP14 Gene in Maize
摘要
Abstract
TCP is a family of transcription factors unique to plants containing bHLH structures,in-volved in the regulation of development and abiotic stress in plant cells.In this study,a significantly down-regulated target gene ZmTCP14 was screened from TCP transcription factor family by fluores-cence quantitative expression analysis under drought stress.Combined with bioinformatics,the bio-logical structure and function prediction analysis of ZmTCP14 gene were carried out,and finally,the ZmTCP14 gene was cloned and a plant overexpression vector was constructed.The results showed that ZmTCP14 was highly correlated with sorghum(Sorghum bicolor),rice(Oryza sativa)and millet(Setaria italica).It contained 20 motifs with highly conserved domains,and the homologous protein alignment was consistent with the phylogenetic tree analysis results.This protein was a class of hy-drophilic proteins located in the nucleus,and contained multiple phosphorylation sites,but no lipo-protein signal peptides and transmembrane structures.Analysis of the promoter element sequences revealed a close relationship between ZmTCP14 gene and stress and light response.Finally,a single target band of 2 267 bp was successfully cloned,and the plant overexpression vector pCAMBIA3301-ZmTCP14-Bar was constructed.This study lays a foundation for the subsequent exploration of the regulatory mechanism of plant drought-related genes.关键词
玉米/TCP转录因子/过表达载体的构建/ZmTCP14/生物信息学分析Key words
maize/TCP transcription factor/construction of overexpression vector/ZmTCP14/bioinformatics analysis分类
农业科技引用本文复制引用
刘桐羽,刘梦彤,周洋洋,逯建宇,曲静,郭树成,关淑艳,马义勇..玉米ZmTCP14基因的筛选、生物信息学分析及植物表达载体的构建[J].吉林农业大学学报,2026,48(2):212-220,9.基金项目
吉林省科技支撑计划项目(20200402023NC,20210302003NC) （20200402023NC,20210302003NC）
