寄生虫与医学昆虫学报2026，Vol.33Issue(1)：25-30,6.DOI:10.3969/j.issn.1005-0507.2026.01.004
2023年北京市淡色库蚊中浓核病毒的鉴定及感染率研究
IDENTIFICATION AND INFECTION RATE OF DENSOVIRUS IN CULEX PIPIENS PALLENS IN BEIJING IN 2023
摘要
Abstract
Objective This study conducted molecular biological identification of the viruses carried by Culex pipiens pallens specimens collected in Shunyi District,Beijing in 2023,and observed the changes in the infection rate of the viruses carried by Cx.pipiens pallens at different collection times.Methods Cx.pipiens pallens were collected using carbon dioxide mosquito traps.The mosquito samples were ground in batches and analyzed by molecular biology technologies.The virus infection rate at different collection times was analyzed statistically.Results 17 strains of Culex pipiens pallens densovirus(CppDNV)were identified from Cx.pipiens pallens samples collected in Shunyi District,Beijing,in 2023.The nucleotide sequence analysis of the virus genome coding region showed that CppDNV was a single-stranded DNA virus with a total length of 3 335 nt,encoding 2 non-structural proteins(NS1,NS2)and 1 capsid protein(VP).The nucleotide(amino acid)sequence lengths of the three proteins were 2 376 nt(791 aa),1 092 nt(363 aa)and 1 071 nt(356 aa),respectively.Phylogenetic analysis showed that CppDNV was located in genus Brevihamaparvovirus.Statistical analysis showed no significant difference in infection rates across collection times(χ2=4.429,P=0.194).Conclusions CppDNV was identified in Cx.pipiens pallens in Beijing,and it was stably maintained in this natural population.关键词
细小病毒/Brevihamaparvovirus属/淡色库蚊/浓核病毒/感染率Key words
Parvovirus/Brevihamaparvovirus/Culex pipiens pallens/Densovirus/Infection rate分类
医药卫生引用本文复制引用
许秀燕,佟颖,张勇,阎婷,朱思捷,李静,刘美德,张洪江,刘婷,李秋红,周小洁..2023年北京市淡色库蚊中浓核病毒的鉴定及感染率研究[J].寄生虫与医学昆虫学报,2026,33(1):25-30,6.基金项目
基于机器学习和气象因素的北京市蚊虫及蚊媒传染病监测预警研究(首发2024-2G-30116) （首发2024-2G-30116）
