寄生虫与医学昆虫学报2026，Vol.33Issue(1)：31-39,9.DOI:10.3969/j.issn.1005-0507.2026.01.005
基于线粒体COⅠ基因分析山东省白纹伊蚊种群遗传多样性
GENETIC DIVERSITY ANALYSIS OF AEDES ALBOPICTUS POPULATIONS IN SHANDONG PROVINCE USING MITOCHONDRIAL MTDNA-COⅠ GENE SEQUENCES
摘要
Abstract
Objective This study aimed to investigate the genetic diversity,differentiation,and population structure of Aedes albopictus across different geographical regions in Shandong Province,and to explore the relationship between genetic diversity and geographical distribution.Methods Between July and August 2024,ten Ae.albopictus populations were sampled from seven cities in Shandong Province.Genomic DNA was extracted from individual mosquitoes,and the mitochondrial cytochrome c oxidase subunit I(COⅠ)gene was amplified using PCR and sequenced.The obtained sequences were verified using BLAST and analyzed with MAFFT,MEGA 11,DnaSP v6.12,Arlequin 3.5,PopART 1.7,STRUCTURE 2.3.4 and the R packages adegenet and vegan to assess genetic diversity and population structure.Results A total of 229 COⅠ sequences(662 bp)was obtained,revealing ten variable sites with no insertions or deletions.The overall base composition showed an AT bias of 67.7%.Haplotype analysis identified 11 haplotypes,with Hap2 being the dominant and most widely distributed haplotype across all populations.Neutrality tests showed significant population expansion only in the Rizhao population.Mantel testing result revealed that geographical distance does not significantly impede gene flow.Overall,genetic differentiation among populations was low,indicating frequent gene flow.When combined with additional samples from Shanghai,Fujian,Guangdong,Yunnan,Hainan and Guangxi,STRUCTURE,UPGMA and DAPC analyses revealed two primary genetic clusters of Ae.albopictus.Conclusions Ae.albopictus populations in Shandong Province exhibit frequent gene flow,low genetic differentiation,and relatively low overall genetic diversity.However,the Rizhao population showed signs of recent expansion,highlighting the need for enhanced surveillance and targeted control measures in this area.关键词
白纹伊蚊/线粒体细胞色素C氧化酶亚基Ⅰ/种群遗传结构/遗传多样性Key words
Aedes albopictus/Mitochondrial cytochrome C oxidase subunit I/Population genetic structure/Genetic diversity分类
医药卫生引用本文复制引用
孟凡进,刘勇,黄欢,马伟博,吴一凡,谭伟龙..基于线粒体COⅠ基因分析山东省白纹伊蚊种群遗传多样性[J].寄生虫与医学昆虫学报,2026,33(1):31-39,9.基金项目
江苏省社发项目(编号:BE2022682) （编号:BE2022682）
江苏省自然科学基金(编号:BK20221196) （编号:BK20221196）
