江苏农业学报2026，Vol.42Issue(3)：433-445,13.DOI:10.3969/j.issn.1000-4440.2026.03.001
水稻萌发期温度胁迫响应的转录调控机制
Transcriptional regulation mechanism of temperature stress response during rice germination stage
摘要
Abstract
Temperature is an important factor affecting rice seed germination and yield formation.A germination ex-periment was conducted under three temperature treatments,including normal temperature(NT,25 ℃),low temperature(LT,15 ℃),and high temperature(HT,37 ℃)with Nipponbare as the test material in this study,and rice seeds cul-tured in dark for 60 h were collected for transcriptome analysis.The results showed that compared with normal temperature treatment,low temperature treatment could significantly delay seed germination,while high temperature treatment accele-rated seed germination.A total of 8 010,3 929 and 9 427 differentially expressed genes were identified in the low temperature and normal temperature comparison group,the normal temperature and high temperature comparison group,and the low temperature and high temperature com-parison group,respectively.Results of KEGG enrichment analysis showed that temperature stress seriously disturbed the carbon and nitrogen balance during seed germination and destroyed the energy and material supply required for seed germination.The plant hormone signal transduction pathway showed a temperature-specific response pattern.Low temperature(LT)interfered with gibberellin metabolism and abscisic acid metabolism,while high temperature(HT)only affected the abscisic acid pathway.Under temperature stress,the ex-pression levels of genes related to jasmonic acid signaling pathway and salicylic acid signaling pathway in germinated rice seeds changed significantly,indicating that temperature stress could activate the immune defensive response of rice seeds.Low temperature could significantly induce ascorbic acid metabolism and aldonic acid metabolism,while high temperature could promote the biosynthesis of terpenoid skeleton and ubiquinone and other terpenoid quinones.The expression levels of several important transcription factor family members changed significantly.These transcription factors can enhance seed stress tolerance by regulating the expression of downstream stress genes.The results of this study systematically revealed the transcriptional regulatory network of rice seeds in response to temperature stress,which are of great significance for cultiva-ting wide-adapted rice varieties.关键词
水稻/萌发/温度胁迫/转录组分析/差异表达基因Key words
rice/germination/temperature stress/transcriptome analysis/differentially expressed gene分类
农业科技引用本文复制引用
李康宁,徐凌璐,宣恒鑫,李程,高梦涛..水稻萌发期温度胁迫响应的转录调控机制[J].江苏农业学报,2026,42(3):433-445,13.基金项目
江苏农林职业技术学院高层次人才科研启动项目(2024rc06) （2024rc06）
国家自然科学基金项目(32402663) （32402663）
中国博士后科学基金第74批面上资助项目(2023M741736) （2023M741736）
