江苏农业学报2026，Vol.42Issue(3)：446-451,6.DOI:10.3969/j.issn.1000-4440.2026.03.002
转抗菌肽基因Ace-AMP-sn抗纹枯病小麦新品系的创制与评价
Creation and evaluation of a new wheat line transformed with antimicrobial peptide gene Ace-AMP-sn resistant to sharp eyespot
摘要
Abstract
Sharp eyespot has become a major soil-borne fungal disease threatening wheat production in China in re-cent years.Breeding disease-resistant varieties is the most economical and effective strategy for controlling this disease.In this study,the T2 generation wheat variety Yangmai 18 transformed with the onion antimicrobial peptide gene Ace-AMP-sn was used as the material.Through successive generations of resistance identification,three T7 transgenic lines E308,E328,and E332 were obtained.Quantitative real-time PCR(qRT-PCR)results showed that the rela-tive expression levels of Ace-AMP-sn in T7 transgenic lines E308,E328,and E332 were significantly higher than those in the recipient variety Yangmai 18(P＜0.05).Results of disease resistance identification demonstrated that the sharp eyespot disease indices of the three T7 transgenic lines were significantly lower than that of Yangmai 18(P＜0.05),while no significant difference was observed compared with the resistant variety CI12633(P＞0.05).These results indicated that the introduction of Ace-AMP-sn could effectively improve resistance to sharp eyespot in wheat.Furthermore,no signifi-cant differences(P＞0.05)were detected between all T7 transgenic lines and Yangmai 18 in key agronomic traits,including heading date,plant height,spike traits,and yield components,suggesting that the introduction of this gene had no adverse effects on main agronomic traits.This study provides new germplasm with outstanding resistance and excellent agronomic traits for the genetic improvement of sharp eyespot resistance in wheat.关键词
小麦纹枯病/抗菌肽/转基因/抗性Key words
wheat sharp eyespot/antimicrobial peptide/transgene/resistance分类
农业科技引用本文复制引用
郭炜,张增艳,吴纪中,张巧凤,祝秀亮,付必胜,蔡瑾,刘颖,翟文玲,吴小有,蔡士宾..转抗菌肽基因Ace-AMP-sn抗纹枯病小麦新品系的创制与评价[J].江苏农业学报,2026,42(3):446-451,6.基金项目
转基因生物新品种培育重大专项(2016ZX08002-001) （2016ZX08002-001）
江苏省农业科技自主创新基金项目[CX(23)3092] （23）
