江苏农业学报2026，Vol.42Issue(3)：464-474,11.DOI:10.3969/j.issn.1000-4440.2026.03.004
棉花纤维长度GhCRK26-1931 SNP分子标记的开发及应用
Development and application of the molecular marker GhCRK26-1931 for cotton fiber length
摘要
Abstract
As a core indicator of cotton fiber quality,fiber length plays a crucial role in accelerating the breeding process through the development of its molecular markers.In this study,a molecular marker(GhCRK26-1931 SNP)asso-ciated with cotton fiber length was developed via localization based on bulked segregant analysis combined with whole-ge-nome sequencing(BSA-Seq),and its application value was systematically evaluated.Competitive allele-specific PCR(KASP)genotyping and phenotypic analysis were conducted on 512 cotton varieties(lines).The results showed that the tested materials exhibited rich genetic diversity,and there were significant phenotypic variations in fiber length among extreme fiber ma-terials of Gossypium hirsutum-Gossypium barbadense backcross populations,G.hirsutum germplasm materials,and G.barbadense germplasm materials,with the coefficient of variation ranging from 3.48％to 9.65％.Cluster analysis based on phenotypic data such as fiber length divided the materials into four major groups.Verification test results indicated that the genotyping results of GhCRK26-1931 SNP molecular marker in the parental Line 9 of the G.hirsutum-G.barbadense backcross populations and Xinhai 16 were completely consistent with the BSA-seq data:Line 9 had the TT genotype,while Xinhai 16 had the CC genotype.Among 60 extreme materials of the G.hirsutum-G.barbadense backcross populations,there was a significant difference in fiber length be-tween the CC genotype(37 materials)and the TT genotype(23 materials)(P＜0.05),and the average fiber length of materials with the TT genotype was shorter than that of materials with the CC genotype.Furthermore,a similar pattern was observed in 305 G.hirsutum materials and 145 G.barbadense materials:the average fiber length of materials with the TT genotype was still shorter than that of materials with the CC genotype(P＜0.05).In conclusion,the GhCRK26-1931 SNP molecular marker can be effec-tively used in the selective breeding for cotton fiber length,and the CC genotype has a positive effect on improving fiber length.This study not only opens up a new avenue for the breeding of high-quality cotton varieties but also provides a reliable tool for marker-assisted selection of cotton fiber quality.关键词
棉花/纤维长度/CRK26基因/KASP-SNP分子标记Key words
cotton/fiber length/CRK26 gene/KASP-SNP molecular marker分类
农业科技引用本文复制引用
李静,庞博,耿世伟,张茹,陈佳林,宋武,马晶晶,李生梅,高文伟..棉花纤维长度GhCRK26-1931 SNP分子标记的开发及应用[J].江苏农业学报,2026,42(3):464-474,11.基金项目
国家重点研发计划子课题项目(2021YFD1900802-4) （2021YFD1900802-4）
新疆维吾尔自治区"天池英才"引进计划项目 （）
新疆维吾尔自治区自然科学基金项目(2025D01B96) （2025D01B96）
2024年度新疆农业职业技术大学资助课题项目(XJNZYBS202401) （XJNZYBS202401）
