江苏农业学报2026，Vol.42Issue(3)：475-484,10.DOI:10.3969/j.issn.1000-4440.2026.03.005
向日葵HaOSCA1.2基因克隆及表达特性分析
Cloning and expression characteristics of HaOSCA1.2 gene in sunflower
摘要
Abstract
The hyperosmolarity-gated calcium-permeable channel family genes(OSCA)play a key regulatory role in plant responses to abiotic stresses.To clarify the molecular characteristics of sunflower HaOSCA1.2 and its response mecha-nism to stresses,this study cloned the HaOSCA1.2 gene.The coding sequence(CDS)of HaOSCA1.2 is 2 322 bp in length,encoding 773 amino acids.The relative molecular weight of the encoded protein is 88 430,with a theoretical isoe-lectric point of 8.81.It contains the RSN1_7TM conserved domain and is a hydrophobic transmembrane protein,whose sec-ondary structure is dominated by α-helices.Evolutionary analysis revealed that HaOSCA1.2 shared high amino acid se-quence similarity(88.93％-89.13％)with OSCA proteins from Tanacetum cinerariifolium,Arctium lappa,and Lactuca sativa,indicating a close phylogenetic relation-ship.Tissue-specific expression analysis revealed that the expression level of HaOSCA1.2 in sunflower leaves was significantly higher than that in roots and stems(P＜0.05).Analysis of abiotic stress response showed that the HaOSCA1.2 gene exhibited rapid and upregulated expres-sion under drought,high salt,and mixed saline-alkali stresses.Protein-protein interaction prediction indicated that HaOSCA1.2 potentially interacted with two members of the DnaJ heat shock protein(HSP40)family.This study clarifies the molecular characteristics and abiotic stress response pat-terns of the sunflower HaOSCA1.2 gene,providing a theoretical basis and genetic resources for analyzing the molecular mechanisms of sunflower stress resistance and for breeding stress-resistant varieties.关键词
向日葵/OSCA1.2基因/表达特性/非生物胁迫Key words
sunflower/OSCA1.2 gene/expression characteristics/abiotic stress分类
农业科技引用本文复制引用
韩志彬,张春,史永军,安琪,彭荣杰,陈阳..向日葵HaOSCA1.2基因克隆及表达特性分析[J].江苏农业学报,2026,42(3):475-484,10.基金项目
内蒙古自治区科技创新重大示范项目(2025ZDSF0014) （2025ZDSF0014）
河套学院高层次人才启动项目(HYRC202319) （HYRC202319）
河套学院科研科技创新团队建设项目(HTKCT-A202405) （HTKCT-A202405）
