军事医学2026，Vol.50Issue(3)：177-181,5.DOI:10.7644/j.issn.1674-9960.2025-00264
抗西尼罗病毒单链抗体与狂犬病毒糖蛋白衍生突变多肽2D3融合蛋白的表达及其功能鉴定
Expression and function of a fusion protein comprising a scFv against West Nile virus and the rabies virus glycoprotein-derived mutant peptide 2D3
摘要
Abstract
Objective To evaluate the ability of mutant 2D3(derived from the rabies virus glycoprotein(RVG)-derived peptide,RDP)to deliver the anti-West Nile virus(WNV)single-chain variable fragment(scFv)into human neuroblastoma SH-SY5Y cells.Methods The cell-penetrating ability of 2D3 was tested using a green fluorescent protein(GFP)reporter system.The anti-West Nile virus single-chain variable fragment-2D3 fusion protein(single-chain variable fragment-2D3,scFv-2D3)was designed and expressed via genetic engineering.The antigen-binding activity and cell penetration into the target cells were assessed using enzyme linked immunosorbent assay(ELISA)and flow cytometry.Results The 2D3 mutant showed stronger neurotropism and could more effectively mediate the uptake of the GFP reporter plasmid into SH-SY5Y cells than the parental RDP.The scFv-RDP could bind to West Nile virus envelope protein domain Ⅲ(WNV EDⅢ)in a concentration-dependent manner and the binding activity was comparable to that of the parental scFv without the penetrating peptide.scFv was detected intracellularly in SH-SY5Y cells treated with scFv-2D3,suggesting that 2D3 could efficiently deliver scFv into neuronal cells.Conclusion 2D3 can more effectively deliver reporter plasmids and the anti-West Nile virus scFv into human neuroblastoma cells.This finding is expected to facilitate subsequent in vivo functional evaluation of the scFv-2D3 fusion protein.关键词
狂犬病毒糖蛋白(RVG)衍生肽/单链抗体/西尼罗病毒/靶向递送Key words
rabies virus glycoprotein(RVG)-derived peptide/single-chain variable fragment/West Nile virus/targeted delivery分类
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吴灵莉,朱婉露,李行舟,沈倍奋,冯健男,吕梦蝶,肖鹤..抗西尼罗病毒单链抗体与狂犬病毒糖蛋白衍生突变多肽2D3融合蛋白的表达及其功能鉴定[J].军事医学,2026,50(3):177-181,5.基金项目
国家自然科学基金面上项目(31771010) （31771010）
