军事医学2026，Vol.50Issue(3)：192-198,7.DOI:10.7644/j.issn.1674-9960.2025-00236
中枢神经系统感染患者脑脊液微生物组特征分析
Microbiome diversity of cerebrospinal fluid in patients with central nervous system infections
摘要
Abstract
Objective To characterize the cerebrospinal fluid(CSF)microbiome in patients with central nervous system(CNS)infections and to compare its diversity with healthy controls using metagenomic next-generation sequencing(mNGS).Methods Forty-six patients with CNS infections were enrolled as the infection group while 16 healthy individuals served as the control group.CSF samples were collected and subjected to mNGS.The sequencing data was processed via host sequence depletion,taxonomic profiling,and alpha/beta diversity analyses.Alpha diversity was assessed using the Chao1,Shannon,Simpson,and Pielou indices while beta diversity was explored using principal coordinates analysis(PCoA)combined with permutational multivariate analysis of variance(PERMANOVA).Results A total of 143 microbial species were identified from 46 sequencing samples.Proteobacteria,Actinobacteria,Firmicutes,and Ascomycota were the predominant phyla.Alpha diversity indices were significantly different between the infection group and control group(Chao1,Shannon,and Simpson,all P<0.05),suggesting altered microbial richness and diversity in CNS infections.Beta diversity analysis revealed a clear separation in community structure between the two groups via PCoA and PERMANOVA(P<0.05).Conclusion The diversity and composition of the CSF microbiome differ significantly between those with CNS infections and healthy individuals,suggesting correlations between CNS infections and CSF microbiome features.关键词
中枢神经系统感染/宏基因组测序/脑脊液/微生物组/多样性/群落结构Key words
central nervous system infections/metagenomic next-generation sequencing/cerebrospinal fluid/microbiome/diversity/community structure分类
医药卫生引用本文复制引用
方小凤,曹英豪,王云飞,崔玉军,范航,李艳君..中枢神经系统感染患者脑脊液微生物组特征分析[J].军事医学,2026,50(3):192-198,7.基金项目
国家重点研发计划(2022YFB3204404,2022YFC2602304) （2022YFB3204404,2022YFC2602304）
