军事医学2026，Vol.50Issue(3)：199-207,9.DOI:10.7644/j.issn.1674-9960.2025-00196
甘露糖修饰的PLGA-PEG纳米粒穿透血脑屏障及其在创伤后应激障碍模型脑部富集研究
Mannose-modified PLGA-PEG nanoparticles penetrating the blood-brain barrier and their brain enrichment in a post-traumatic stress disorder model
谢丽斯 1陈蓉龙 1陈雪琴 2林莉 2刘娟 2宋春雪 2张超3
作者信息
- 1. 军事科学院军事医学研究院,北京 100850||中山大学孙逸仙纪念医院医学研究中心,广东省恶性肿瘤表观遗传学与基因调控重点实验室,广州 510120
- 2. 中山大学孙逸仙纪念医院医学研究中心,广东省恶性肿瘤表观遗传学与基因调控重点实验室,广州 510120
- 3. 军事科学院军事医学研究院,北京 100850
- 折叠
摘要
Abstract
Objective To design and synthesize a mannose-modified PLGA-PEG nanodrug delivery system capable of brain-targeting accumulation and blood-brain barrier(BBB)permeation.Methods bEnd.3 mouse vascular endothelial cells were used to establish a monolayer cell model using Transwell chambers.The barrier integrity was evaluated by measuring the transendothelial electrical resistance before an in vitro BBB model was established.A post-traumatic stress disorder model was established to find out about the in vivo BBB permeability of nanomaterials.In the in vitro experiment,nanomaterials were delivered to BV2 cells while in the in vivo experiment,nanomaterials were injected into the tail vein of C57BL/6 mice.The ability of nanomaterials to penetrate the blood-brain barrier was detected by a spectrophotometer,laser confocal microscopy and flow cytometry.The distribution and rate of brain enrichment of nanomaterials were dynamically monitored by a small animal in vivo near-infrared fluorescence imaging system.Results In vitro experiments showed that the PPM system loaded with a fluorescent molecule,purpurin 18(P18),could effectively penetrate the BBB with a fluorescence permeability of 7%.The system was capable of both prolonged retention in the brain with a brain accumulation rate of 30%,and significant brain enrichment in a post-traumatic stress disorder(PTSD)mouse model with an enrichment efficiency of 54%.Conclusion The newly developed BBB-penetrating nano-delivery system can ensure effective BBB penetration and targeted delivery in PTSD mouse models,with significant implications and translational potential for neuropharmaceutical development.关键词
血脑屏障/透血脑屏障纳米递送系统/创伤后应激障碍/甘露糖/聚乳酸-羟基乙酸共聚物-聚乙二醇Key words
blood-brain barrier/blood-brain barrier penetrating nano-delivery system/post-traumatic stress disorder/mannose/poly(lactic-co-glycolic acid)-polyethylene glycol分类
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谢丽斯,陈蓉龙,陈雪琴,林莉,刘娟,宋春雪,张超..甘露糖修饰的PLGA-PEG纳米粒穿透血脑屏障及其在创伤后应激障碍模型脑部富集研究[J].军事医学,2026,50(3):199-207,9.
