菌物学报2026，Vol.45Issue(4)：116-133,18.DOI:10.13346/j.mycosystema.250259
香菇LeATG基因家族及其在菌丝转色过程中的表达
Members of the LeATG gene family and their expression during mycelial colouring process of Lentinula edodes
摘要
Abstract
The autophagy-related gene family members in Lentinula edodes(LeATG family members)were identified based on the whole genome data of L.edodes using homologous alignment methods,and their biological information was analyzed.The expression patterns of these family members during mycelial colouring process were analyzed by combining transcriptome sequencing data and RT-qPCR.The results showed that 29 LeATG family members belonging to 23 classes of autophagy-related genes were identified in the L.edodes genome.Chromosomal localization analysis revealed that they were mainly distributed on chromosomes 4 and 1;no distribution was detected on chromosome 9.The full length of LeATG family members ranges from 595 to 6 772 bp.The length of protein sequence encoded by the LeATG gene ranged from 127 to 1 998 aa,with relative molecular weights of 14.8-219.7 kDa and theoretical isoelectric points of 4.27-9.51.Except for LeATG9,LeATG22,and LeATG27,the other 24 LeATG proteins contained no transmembrane domains.The phylogenetic analysis showed that the 29 LeATG proteins clustered into three evolutionary branches,and proteins of the same class were dispersed across different sub-branches,suggesting significant differences in protein structure.Transcriptome data analysis and RT-qPCR revealed differential expression patterns of LeATG genes before and after mycelial colouring process:2 LeATG genes were highly expressed before browning,5 were highly expressed after colouring.It was speculated that these 7 genes might be involved in regulating the mycelial colouring process.This study systematically identified the LeATG gene family in L.edodes,and preliminarily revealed their expression regulation patterns during mycelial colouring process,laying an important foundation for further analysis of the molecular mechanism of the LeATG gene during mycelial colouring process of L.edodes.关键词
香菇/自噬相关基因/菌丝转色/基因表达Key words
Lentinula edodes/autophagy-related genes/mycelial colouring/gene expression引用本文复制引用
胡梦佳,高琪,范阳阳,严冬,王守现,刘宇,宗宪春,刘媛媛..香菇LeATG基因家族及其在菌丝转色过程中的表达[J].菌物学报,2026,45(4):116-133,18.基金项目
国家自然科学基金(32202568) （32202568）
北京市农林科学院植物保护研究所改革与发展项目(GGYFZ202512) （GGYFZ202512）
现代农业产业技术体系北京市食用菌创新团队(BAIC03) This work was supported by the National Natural Science Foundation of China(32202568),the Reform and Development Project,Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences(GGYFZ202512),and the Beijing Innovation Consortium of Agriculture Research System(BAIC03). （BAIC03）
