菌物学报2026，Vol.45Issue(4)：134-144,11.DOI:10.13346/j.mycosystema.250251
腺苷酸基琥珀酸合成酶负介导罗伯茨绿僵菌致病力
Adenylosuccinate synthase negatively controls Metarhizium robertsii pathogenicity
摘要
Abstract
Adenylosuccinate synthetase(ADSS)plays an important role in cellular energy metabolism and signal transduction.However,ADSS had not been reported in entomopathogenic fungi.In this study,function of ADSS family protein MrADSS-1 to the growth,stress resistance and pathogenicity in the entomopathogenic fungus Metarhizium robertsii was characterized by investigating the protein subcellular localization and gene disruption,complementation and over-expression.Results showed that MrADSS-1 is exclusively localized within the vacuole.Disruption of the MrADSS-1 resulted in marked reduction of radial growth of the fungus on maltose medium and a significant decrease in UV-B tolerance,but unexpectedly enhanced thermotolerance.Bioassays revealed that loss of MrADSS-1 led to a pronounced increase in appressorium formation and penetration efficiency through cicada wings,resulting in elevated pathogenicity toward Galleria mellonella larvae.Conversely,over-expression of MrADSS-1 attenuated fungal virulence.These findings demonstrated that MrADSS-1 functions as a repressor of both thermotolerance and virulence in M.robertsii.关键词
腺苷酸基琥珀合成酶/罗伯茨绿僵菌/亚细胞定位/逆境胁迫/致病力Key words
adenylosuccinate synthase/Metarhizium robertsii/subcellular localization/abiotic stress/pathogenicity引用本文复制引用
王乐陈,郭世悦,赵雨桐,董媛媛,吴文婧,任言,郭复明,王玉龙..腺苷酸基琥珀酸合成酶负介导罗伯茨绿僵菌致病力[J].菌物学报,2026,45(4):134-144,11.基金项目
合肥市自然科学基金(202312) （202312）
安徽省大学生创新创业训练计划(S202410364133,X202410364462,X202410364493) This work was supported by the Hefei Municipal Natural Science Foundation(202312)and the Anhui College Student Innovation and Entrepreneurship Training Program(S202410364133,X202410364462,X202410364493). （S202410364133,X202410364462,X202410364493）
