菌物学报2026，Vol.45Issue(4)：160-171,12.DOI:10.13346/j.mycosystema.250263
雪腐核盘菌ClyA_Cry6Aa-like成孔毒素蛋白功能
Functions of ClyA_Cry6Aa-like pore-forming toxin proteins in Sclerotinia nivalis
摘要
Abstract
Pore-forming toxins(PFTs)are present in fungi,but their functions remain poorly understood.In this study,analysis of differentially expressed genes between sclerotia and mycelia of Sclerotinia nivalis revealed that genes encoding pore-forming toxin proteins were highly expressed in sclerotia.Further proteomic profiling by mass spectrometry confirmed the abundant accumulation of these proteins in sclerotia.Sequence and structural analyses indicated that three pore-forming toxin proteins SnCry6Aa1,SnCry6Aa2,and SnCry6Aa3 were encoded by S.nivali SnTB1.All of which contain the conserved ClyA_Cry6Aa-like PFTs domain and exhibit the characteristic three-dimensional structure of α-PFTs.The recombinant SnCry6Aa1 and SnCry6Aa2 were successfully expressed and purified using a prokaryotic system,and their hemolytic activity and nematidal/insecticidal toxicity were subsequently assessed.The results showed that SnCry6Aa1 exhibited significant hemolytic activity at concentrations over 6.25 ng/mL under acidic conditions,whereas no similar activity was detected for SnCry6Aa2.Neither SnCry6Aa1 nor SnCry6Aa2 showed notable toxicity against Caenorhabditis elegans,Helicoverpa armigera,Spodoptera litura,Grapholita molesta,or Bradysia odoriphaga.However,both displayed low but detectable insecticidal activity against larvae of Pnyxia scabiei,with corrected mortality rates of 14%and 17%,respectively.This study is the first to identify the presence of ClyA_Cry6Aa-like PFTs at high abundance in the sclerotia of S.nivalis and provides initial insights into their biological roles,laying a foundation for further functional studies and potential applications of these proteins.关键词
雪腐核盘菌/菌核/ClyA_Cry6Aa-like成孔毒素蛋白/红细胞裂解/杀虫活性Key words
Sclerotinia nivalis/sclerotium/ClyA_Cry6Aa-like pore-forming toxin protein/erythrocyte lysis/insecticidal efficiency引用本文复制引用
曹燕霞,耿士帅,张恒嘉,李文杰,刘健,杨舒涵,王思钧,范三红,胡小平..雪腐核盘菌ClyA_Cry6Aa-like成孔毒素蛋白功能[J].菌物学报,2026,45(4):160-171,12.基金项目
国家现代农业(小麦)产业技术体系项目(CARS-03-37) （小麦）
西北农林科技大学作物病虫害监测与治理创新团队项目(XYTD2023-04) This work was supported by the China Agriculture Research System(CARS-03-37)and the Innovation Team Project for Crop Disease and Pest Monitoring and Management of Northwest A&F University(XYTD2023-04). （XYTD2023-04）
