江西农业学报2026，Vol.38Issue(4)：8-13,6.DOI:10.19386/j.cnki.jxnyxb.2026.04.002
培养基优化与基因型筛选协同提升裸大麦花药培养效率的研究
Research on Genotype and Culture Medium Optimization for Improving Anther Culture Efficiency of Naked Barley
摘要
Abstract
In order to establish an efficient anther culture system of naked barley and explore the synergistic effects of medium optimization and genotypes,this study conducted anther culture using two medium combinations(M1 and M2)with five barley materials.And using the optimized medium combination M2,49 naked barley materials were cultured in vitro to explore the synergistic effect of medium optimization and genotype screening on improving the efficiency of naked barley anther culture.The results showed that:(1)The combination M2 effectively enhanced the anther culture efficiency of naked barley.The callus induction rate,green seedling differentiation rate and average anther culture efficiency of M2 were increased by 9.73,14.28 and 2.76 percentage points comparing with M1,respectively.(2)In the combination M2,the callus was induced from 34 samples.Among them,the hybrid materials performed significantly better than homozygous materials.Line 22-73-1-1 showed the best performance among all the materials,with a callus induction rate of 46.91%,green seedling differentiation rate of 72.37%,and anther culture efficiency of 33.95%.(3)The anther culture efficiency of naked barley varies greatly among different genotypes,showing genotype-dependent characteristics.Flow cytometry analysis showed that diploids accounted for 80%and haploids for 20%in the regenerated plants,without multiploids plants.关键词
裸大麦/花药培养/基因型依赖/培养基优化Key words
Naked barley/Anther culture/Genotype dependence/Medium optimization分类
农业科技引用本文复制引用
刘海翠,李赢,石晓旭,石吕,韩笑,严旖旎,魏亚凤..培养基优化与基因型筛选协同提升裸大麦花药培养效率的研究[J].江西农业学报,2026,38(4):8-13,6.基金项目
南通市科技项目(MS2024049) （MS2024049）
江苏沿江地区农业科学研究所学科基金[YJXK(2023)005]. （2023）
