江西农业学报2026，Vol.38Issue(4)：14-21,8.DOI:10.19386/j.cnki.jxnyxb.2026.04.003
百香果NBS-LRR类抗病基因同源序列的克隆与分析
Cloning and Analysis of NBS-LRR Resistance Gene Analogs of Passion Fruit
摘要
Abstract
Discovering endogenous plant resistance genes is a critical preliminary foundation for breeding disease-resistant varieties.In this study,degenerate primers were designed based on the conserved domains of known plant NBS-LRR resistance genes,and Passiflora edulis f.flavicarpa'Qinguo 9'was used as the experimental material to clone NBS-LRR resistance gene analogs(RGA)from its genome.The results showed that the NBS-LRR resistance genes of this cultivar could be divided into two types:TIR type and non-TIR type.Among them,there were 12 TIR-NBS-LRR subtypes(designated QRGA1 to QRGA12)and 4 non-TIR-NBS-LRR subtypes(designated QRGA13 to QRGA16).Phylogenetic analysis revealed that QRGA1～QRGA12 clustered with the reported tobacco TMV-resistance gene N and flax resistance gene L6,with a homology of 86%.In contrast,QRGA13～QRGA16 clustered with rice bacterial blight-resistance gene Xa-1 and Arabidopsis pseudomonas syringae-resistance gene RPM1,with a homology of 63%.Notably,the amino acids encoded by QRGA1～QRGA12 showed high similarity to TMV-resistant proteins and RPV1-resistant proteins from multiple plant species,indicating their potential research and application value.The findings of this study provide support for the discovering and subsequent application of resistance genes in Passiflora edulis.关键词
百香果/NBS-LRR/抗病基因/同源序列/序列分析Key words
Passion fruit/NBS-LRR/Resistance gene/Homology sequence/Sequence analysis分类
农业科技引用本文复制引用
杨翠凤,滕峥,侯宪斌,牛俊乐,李锐霞,刘世恒,莫罗燕..百香果NBS-LRR类抗病基因同源序列的克隆与分析[J].江西农业学报,2026,38(4):14-21,8.基金项目
广西自然科学基金项目(2023GXNSFBA026046). （2023GXNSFBA026046）
