江西中医药大学学报2026，Vol.38Issue(2)：79-85,7.DOI:10.20140/j.2095-7785.2026.02.20
基于UHPLC-Q-Exactive-Orbitrap MS/MS和网络药理学探讨金线莲抗抑郁有效成分及作用机制研究
The Effective Ingredients and Mechanism of Anoectochilus Roxburghii in Treating Depression Based on UHPLC-Q-Exactive-Orbitrap MS and Network Pharmacology
摘要
Abstract
Objective:To explore the pharmacological material basis and potential mechanism of action of Anoectochilus roxburghii in the treatment of depression.Methods:Ultra-high performance liquid chromatography-quadrupole-Orbitrap high-resolution mass spectrometry(UHPLC-Q-Exactive-Orbitrap MS)was used to analyze the chemical components and blood components of Anoectochilus roxburghii water extract.The target sites of the blood components were obtained from the PubChem and Swiss Target Prediction databases.The target sites of the disease were obtained from the GeneCards,Therapeutic Target,and OMIM databases.The protein-protein interaction(PPI)network was constructed using the STRING platform.The intersection targets were analyzed for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment using the DAVID database.Results:A total of 42 compounds were identified in the water extract of Anoectochilus roxburghii,and 14 blood components were identified in the plasma of rats after intragastric administration.There were 265 intersection targets between the disease and the blood components,among which 53 were key targets,mainly involving IL-6,AKT1,SRC,STAT3,PPARG,etc.GO functional enrichment and KEGG pathway analysis showed that Anoectochilus roxburghii may exert its therapeutic effect on depression through pathways such as cancer,regulation of TRP channels by inflammatory mediators,neuroactive ligand-receptor interaction,5-hydroxytryptamine(serotonin)synaptic signaling pathway,VEGF signaling pathway,HIF-1 signaling pathway,PI3K-Akt signaling pathway,and FoxO signaling pathway.Conclusion:The active components such as anoectochiluside,apigenin,and 5-hydroxymethylfurfural in Anoectochilus roxburghii may exert its therapeutic effect on depression through pathways such as cancer,regulation of TRP channels by inflammatory mediators,neuroactive ligand-receptor interaction,5-hydroxytryptamine(serotonin)synaptic signaling,VEGF signaling,HIF-1 signaling,PI3K-Akt signaling,and FoxO signaling,as well asgenes such as IL-6,AKT1,SRC,and STAT3.关键词
金线莲/抑郁/入血成分/网络药理学/机制研究Key words
Anoectochilus Roxburghii/Depression/Blood Components/Network Pharmacology/Mechanism Rresearch分类
医药卫生引用本文复制引用
雷颖,张海燕,黄慧辰,刘燕燕,杨明..基于UHPLC-Q-Exactive-Orbitrap MS/MS和网络药理学探讨金线莲抗抑郁有效成分及作用机制研究[J].江西中医药大学学报,2026,38(2):79-85,7.基金项目
国家自然科学基金项目(U21A20414). （U21A20414）
