林产化学与工业2026，Vol.46Issue(2)：39-46,8.DOI:10.20195/j.issn.0253-2417.2025146
木质素模型物/CNC的Pickering乳液制备及其抗肿瘤活性
Preparation of Lignin Model Compounds/CNC Pickering Emulsion and Its Anti-tumor Activity
摘要
Abstract
Based on the synthesis of lignin dehydrogenation polymer(DHP),an oil-in-water(O/W)Pickering emulsion was prepared using cyclopentanone containing DHP as the oil phase,deionized water as the aqueous phase,and cellulose nanocrystals(CNC)as solid particle stabilizers.Using the Pickering emulsion,a novel drug delivery system was developed.The particle size,stability,and rheological properties of the Pickering emulsion were comprehensively characterized with Fourier-transform infrared spectroscopy(FT-IR),size-exclusion chromatography combined with multi-angle light scattering(SEC-MALS),Malvern laser particle size analyzer,and ultraviolet-visible spectrophotometry.Results showed that the emulsion had an average particle size of approximately 430 nm and exhibited good stability.The drug loading capacity of the Pickering emulsion was 24.7％±2.0％,with an encapsulation efficiency of 80.0％±1.8％.In vitro experiments demonstrated that the DHP-containing CNC-Pickering emulsion remained stable in simulated lumen juice but underwent phase separation(i.e.,emulsion breakdown)in simulated colonic juice.After breakdown,the released DHP was evaluated for antitumor activity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)colorimetric assay.The results indicated significant inhibitory effects against human colon cancer HCT116 cells,with a half-maximal inhibitory concentration(IC50)of 186.97 mg/L.Thus,the lignin model compounds/CNC-based Pickering emulsion enables targeted drug release under enzyme-responsive conditions to achieve the therapeutic effect on colon cancer.关键词
木质素模型物/纤维素纳米晶体/Pickering乳液/靶向释放/抗癌Key words
lignin model compound/nanocellulose crystal/Pickering emulsion/targeted release/anti-cancer分类
化学化工引用本文复制引用
谢益民,赵伯轩,王志..木质素模型物/CNC的Pickering乳液制备及其抗肿瘤活性[J].林产化学与工业,2026,46(2):39-46,8.基金项目
国家自然科学基金项目资助(21878070) （21878070）
湖北省高等学校优秀中青年科技创新团队计划项目(T201205) （T201205）
