临床口腔医学杂志2026，Vol.42Issue(4)：208-212,5.DOI:10.3969/j.issn.1003-1634.2026.04.005
负载SNAP的3D打印支架制备及其促成骨作用研究
Fabrication of 3D scaffolds loaded with SNAP and its osteogenic activity
摘要
Abstract
Objective:To develop a S-nitroso-N-acetyl-penicillamine(SNAP)loaded 3D printed scaffold integrating a three-dimensional porous architecture,bioactive signaling,and antibacterial properties,aiming to achieve efficient bone regen-eration while preventing postoperative infections.Methods:Hydrogel scaffolds were fabricated utilizing digital light processing(DLP)technology and loaded with nitric oxide(NO)donor,SNAP.Scaffold morphology and NO release kinetics were subse-quently characterized.Bone marrow mesenchymal stem cells(BMSCs)and MC3T3-E1 cells were employed to evaluate the biocompatibility and in vitro pro-osteogenic capacity of the scaffolds.Furthermore,the antibacterial efficacy of the scaffolds was assessed using the agar plate spreading method.Results:The successfully fabricated SNAP loaded 3D printed scaffolds exhibited a sustained release of NO for at least 3 days.Compared to the control group,these scaffolds effectively inhibited the proliferation of pathogenic bacteria and significantly upregulated the expression of osteogenic genes.Conclusion:This study demonstrates that the SNAP-loaded 3D printed scaffold provides sustained NO release,facilitating simultaneous antibacterial and pro-osteogenic effects.By integrating bone repair capabilities with infection prophylaxis,this dual-functional scaffold holds promising clinical potential for application in bone regeneration.关键词
3D打印/水凝胶支架/一氧化氮/骨再生/抗菌Key words
3D printing/Hydrogel scaffold/Nitric oxide/Bone regeneration/Antibacterial分类
医药卫生引用本文复制引用
刘蓝阳,史云松,孙玮..负载SNAP的3D打印支架制备及其促成骨作用研究[J].临床口腔医学杂志,2026,42(4):208-212,5.基金项目
国家重点研发计划项目(2023YFB4604705) （2023YFB4604705）
