临床输血与检验2026，Vol.28Issue(2)：207-215,9.DOI:10.3969/j.issn.1671-2587.2026.02.009
脐带血随储存时间红细胞损伤变化与微生物安全性评估
Evaluation of Red Blood Cell Damage and Microbiological Safety of Whole Cord Blood during Storage
彭梓睿 1张普山 2孟擎擎 3柳艳丽 4刘玉梅 5娄琦 6袁海云 1温树生1
作者信息
- 1. 广东省心血管病研究所,广东省人民医院,广东省医学科学院心外科,广东 广州 510080||广东省华南结构性心脏病重点实验室,广东 广州 510080
- 2. 广东省人民医院输血科,广东 广州 510080
- 3. 广东省人民医院体外循环科,广东 广州 510080
- 4. 广东省人民医院产科,广东 广州 510080
- 5. 广东省人民医院新生儿科,广东 广州 510080
- 6. 广东省人民医院体外循环科,广东 广州 510080||广东省华南结构性心脏病重点实验室,广东 广州 510080
- 折叠
摘要
Abstract
Objective To evaluate the red blood cell stability and microbiological safety of umbilical cord blood(UCB)stored in citrate phosphate dextrose adenine-1(CPDA-1)anticoagulant preservative solution at 2～6 ℃ for up to 35 days,providing experimental evidence for its use in predeposit autologous transfusion during neonatal cardiac surgery and other clinical scenarios.Methods Within an integrated prenatal-postnatal diagnosis and treatment model for congenital heart disease,UCB samples were collected from 60 neonates prenatally diagnosed with CHD.The samples were randomly divided into five groups.After storage for 7,14,21,28,and 35 days,routine blood counts,electrolytes,free hemoglobin,hemolysis rate,and microbial contamination were measured.Results There were no significant baseline differences among groups before storage.During storage,red blood cell count,hemoglobin,hematocrit,platelet count,and sodium and calcium ion concentrations showed no significant changes.Potassium,free hemoglobin,and hemolysis rate all increased significantly with prolonged storage time(P＜0.05).Post-hoc analysis revealed that potassium level rose steadily from day 7 onwards,while free hemoglobin and hemolysis rate increased significantly after 21 days of storage.Bacterial contamination with Escherichia coli occurred in 1 case(1.7％)among all samples.Conclusion Under standardized collection and storage protocols,UCB stored in CPDA-1 anticoagulant preservative solution for up to 35 days showed progressive potassium accumulation and hemolysis,yet key quality indicators remained within acceptable ranges and microbiological safety was controllable.This study supports that UCB represents a safe and feasible autologous blood source for neonates,particularly those with congenital heart disease requiring time-limited surgery.关键词
脐带血/CPDA-1保存液/预存式自体输血/溶血率Key words
Umbilical cord blood/CPDA-1 anticoagulant preservative solution/Predeposit autologous transfusion/Hemolysis rate分类
医药卫生引用本文复制引用
彭梓睿,张普山,孟擎擎,柳艳丽,刘玉梅,娄琦,袁海云,温树生..脐带血随储存时间红细胞损伤变化与微生物安全性评估[J].临床输血与检验,2026,28(2):207-215,9.
