辽宁中医杂志2026，Vol.53Issue(4)：215-219,后插9-后插10,7.DOI:10.13192/j.issn.1000-1719.2026.04.050
肺不藏魄不寐模型大鼠相关脏器Bcl-2、Bax、Caspase-3表达水平研究
Expression Levels of Caspase-3,Bcl-2 and Bax in Related Organs of Insomnia Rats with Lung Not Storing Corporeal Soul
摘要
Abstract
Objective To investigate the expression levels of Bcl-2,Bax and Caspase-3 in the related organs of insomnia rats with lung not storing corporeal soul,so as to provide a basic experimental basis for the traditional Chinese medicine(TCM)classification of the five spirits of insomnia.Methods According to the random number table method,16 SPF male SD rats were di-vided into large platform control group and lung not storing corporeal soul group,with 8 rats in each group.The large platform con-trol group was used to establish the water environment large platform method,and the lung not storing corporeal soul group was used to establish the model by the water environment small platform method.The general condition of the rats in the two groups was monitored and the water maze experiment was performed.HE was used to observe the pathological changes of lung tissues of the two groups,and immunohistochemistry(IHC)method and Western Blot(WB)were used to detect the expression levels of Caspase-3,Bcl-2 and Bax in the brain and lung tissues of the two groups.Results Compared with those in the control group,the general situation showed that the rats in the lung not storing corporeal soul group gradually became depressed,their hair was dull,there was hair loss from time to time,and their diet gradually deteriorated(P＜0.05).HE staining showed disordered alveolar structure,alveolar epithelial cell edema,widened alveolar septum and more lymphocyte and plasma cell infiltrates.IHC method showed that the expression of Bel-2 in brain tissue and lung tissue decreased(P＜0.05),and the expressions of Bax and Caspase-3 increased(P＜0.05).WB results showed that the expression of Bcl-2 in brain tissue and lung tissue in lung tissue was significantly decreased(P＜0.01),the expressions of Bax and Caspase-3 increased(P＜0.05).Conclusion The changes of apoptosis in brain and lung tissues are related to the occurrence of lung not storing corporeal soul.关键词
中医不寐五神分型/肺不藏魄/Bcl-2/Bax/Caspase-3Key words
traditional Chinese medicine classification of five spirits of insomnia/lung not storing corporeal soul/Bcl-2/Bax/Caspase-3分类
医药卫生引用本文复制引用
王慧,张星平,闫德祺,陈旭,吴金鸿,贾宏林..肺不藏魄不寐模型大鼠相关脏器Bcl-2、Bax、Caspase-3表达水平研究[J].辽宁中医杂志,2026,53(4):215-219,后插9-后插10,7.基金项目
国家自然科学基金项目(82160873,81960837,81560762,81260526) （82160873,81960837,81560762,81260526）
新疆维吾尔自治区自然科学基金重点项目(2022D01D48) （2022D01D48）
全国名老中医药专家传承工作室项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
国家中医药管理局"十四五"高水平中医药重点学科项目(国中医药人教函[2023]85号) （国中医药人教函[2023]85号）
新疆"十四五"中医学重点学科项目(新教函[2022]112号) （新教函[2022]112号）
昌吉州科技计划项目(2025S03-06) （2025S03-06）
