丽水学院学报2026，Vol.48Issue(2)：1-14,14.DOI:10.3969/j.issn.2095-3801.2026.02.001
荧光碳点的绿色合成及其对镉胁迫下豌豆幼苗的毒性缓解效应
Green Synthesis of Fluorescent Carbon Dots and Their Mitigation Effects on Cadmium-Stress Toxicity in Pea Seedlings
摘要
Abstract
The pollution of heavy metal cadmium(Cd2+)seriously threatens plant growth,and there is an urgent need to develop efficient strategies for detecting and mitigating the toxicity of cadmium.This study synthesized a type of carbon dots(CDs)with selective fluorescence response to Cd2+via a calcination method using 5-amino-1,10-phenanthroline and salicylic acid as raw materials.The fluorescence intensity of CDs exhibited a good linear relationship with Cd2+concentration in the range of 0-100 µmol/L,with a detection limit as low as 0.3 µmol/L.Further experiments with pea seedlings revealed the stress-mitigating effects of CDs.The study found that under 400 mg/L Cd²⁺ stress,exogenous application of 0.5 mg/mL CDs reduced Cd2+accumulation in roots and stems by 48.2%and 72.4%,respectively,while significantly restoring the root length,shoot length,and biomass.Mechanistic studies demonstrated that CDs effectively mitigated Cd2+-induced oxidative damage and physiological metabolic disorders by enhancing chlorophyll levels,optimizing photosynthetic parameters,boosting antioxidant enzyme activity,and promoting carbohydrate accumulation.This study not only provides a novel approach for highly sensitive Cd2+detection but also reveals the synergistic role of CDs in reducing heavy metal uptake and improving stress resistance in plants,offering theoretical foundation and technical support for its application in environmental remediation and agricultural production.关键词
5-氨基-1,10-邻二氮菲/水杨酸/碳点/豌豆幼苗/镉胁迫Key words
5-amino-1,10-phenanthroline/salicylic acid/carbon dots/pea seedlings/cadmium stress分类
数理科学引用本文复制引用
汤素雷,王慕华,冯丽萍,徐雅兰,孙晓丽..荧光碳点的绿色合成及其对镉胁迫下豌豆幼苗的毒性缓解效应[J].丽水学院学报,2026,48(2):1-14,14.基金项目
国家自然科学基金面上项目"亲水印迹-比率荧光耦合技术用于双酚类环境内分泌干扰物的可视化检测"(22076072) （22076072）
