林业科学研究2026，Vol.39Issue(2)：24-36,13.DOI:10.12403/j.1001-1498.20250129
一株解磷真菌对毛竹根系生长及磷素利用的影响
The Effect of a Phosphorus-solubilizing Fungus on the Root Growth and Phosphorus Utilization of Phyllostachys edulis
摘要
Abstract
[Objective]To explore how the symbiosis between the phosphorus-solubilizing fungus PDRF3(Lecanicillium huhutii)and P.edulis promotes the growth of P.edulis under phosphorus-limited conditions in southern red soil regions.[Methods]Pot experiments were conducted under controlled conditions,with different phosphorus sources(calcium phytate and tricalcium phosphate),phosphorus concentration levels(2.5～25.0 mg·kg-1),inoculation frequencies(5 times),and fungal treatments(PDRF3 inoculation:+PDRF3;non-inoculated control:-PDRF3).The colonization of PDRF3 in P.edulis roots was quantified using qPCR technology.The effects of each treatment on the growth indices of P.edulis were analyzed.Weighted cor-relation analysis,range analysis,and response surface methodology were employed to screen and optim-ize the optimal infection conditions,and the optimized conditions were then used in a subsequent con-trolled pot experiment to explore the effects of the PDRF3-P.edulis symbiotic relationship on root growth and phosphorus utilization under different phosphorus conditions.[Results](1)PDRF3 rapidly established a symbiotic relationship with P.edulis,promoting increases in seedling height,root growth,internal phos-phorus content,and soil available phosphorus content;(2)the optimal infection conditions were determ-ined to be two inoculations at a phosphorus concentration of 10 mg·kg-1;(3)the PDRF3 symbiosis signific-antly enhanced root activity and phosphorus content in P.edulis(p<0.05),with maximum increases of 199.60%and 210.48%,particularly under low-phosphorus conditions(2.5 mg·kg-1,5 mg·kg-1;p<0.05).[Conclusion]The rapid establishment of the symbiotic relationship between PDRF3 and P.edulis im-proves root activity,promote root elongation,effectively alleviate soil phosphorus limitations,and en-hances P.edulis growth.关键词
解磷真菌PDRF3/毛竹/磷素限制/根系生长Key words
phosphorus-solubilizing fungi PDRF3/Phyllostachys edulis/phosphorus limitations/root growth分类
农业科技引用本文复制引用
谯晓阳,李键,熊佳玉,杨胜钰,马文睿,贺鑫妍,林若涵,陈禹霖,陈思晗,张雪翠..一株解磷真菌对毛竹根系生长及磷素利用的影响[J].林业科学研究,2026,39(2):24-36,13.基金项目
国家自然科学基金(32071753) （32071753）
