林业科学研究2026，Vol.39Issue(2)：105-115,11.DOI:10.12403/j.1001-1498.20250193
沙棘MADS-MIKC基因家族的全基因组鉴定及表达分析
Genome-wide Identification and Expression Analysis of the MADS-MIKC Gene Family in Sea Buckthorn(Hippophae rhamnoides L.)
摘要
Abstract
[Objective]This study aimed to identify members of the MADS-MIKC gene family in sea buck-thorn,and to analyze their physicochemical properties,evolutionary relationships,gene structures and ex-pression patterns,thereby providing a molecular basis for breeding high-quality sea buckthorn germplasm resources and elucidating regulatory mechanisms underlying fruit development.[Method]Using the labor-atory's sea buckthorn genome data,bioinformatics methods were used to identify the members of the gene family,and combined with transcriptome sequencing and RT-qPCR to verify the expression patterns of the genes during the development of sea buckthorn pulp and seeds.[Result]A total of 55 MADS-MIKC gene family members were identified in the sea buckthorn genome and divided into 11 subfamilies,all genes contained introns.Promoter analyses indicated that most genes harboured multiple cis-acting elements as-sociated with responses to abscisic acid,auxin,and gibberellin.The MADS-MIKC gene family was un-evenly distributed across 12 chromosomes,and segmental duplication appeared to be the primary driver of family expansion.Expression heatmap and RT-qPCR analysis showed that the gene family exhibited signi-ficant expression differences in different developmental stages and tissues:the SEP and SHP subfamilies were highly expressed in pulp and seeds,and RT-qPCR verified the reliability of the transcriptome data.[Conclusion]The sea buckthorn MADS-MIKC gene family has tissue-and stage-specific regulatory roles in the process of fruit development,which provides a theoretical basis for analyzing the fruit ripening mech-anism of sea buckthorn and molecular breeding.关键词
沙棘/MADS-MIKC/基因家族/生长发育/表达量分析Key words
sea buckthorn/MADS-MIKC/gene family analysis/growth and development/expression analysis分类
农业科技引用本文复制引用
张岩,李晶,张滨,杨明鹤,丁健,阮成江,鹿梅,赵博文,吕庆鑫,黄瑜,崔灿..沙棘MADS-MIKC基因家族的全基因组鉴定及表达分析[J].林业科学研究,2026,39(2):105-115,11.基金项目
国家自然科学基金项目(32371913) （32371913）
辽宁省科技计划联合计划(基金)项目(2023JH2/101700242) （基金）
辽宁省"兴辽英才计划"项目(XLYC2203001) （XLYC2203001）
水利部沙棘开发管理中心技术委托项目(2025-kj-020)、中央高校基本科研业务费资助(044420250077) （2025-kj-020）
大连民族大学2025年研究生创新项目(0113202201). （0113202201）
