木材科学与技术2026，Vol.40Issue(1)：27-40,14.DOI:10.12326/j.2096-9694.2025122
基于挥发性物质化学指纹方法识别楠属和润楠属木材研究
Species-Level Identification of Phoebe and Machilus Wood Based on Volatile Compound Chemical Fingerprinting
摘要
Abstract
To investigate the feasibility of species-level identification of Phoebe and Machilus wood based on volatile compound chemical fingerprinting,four species,namely P.hui,P.bournei,M.pauhoi,and M.leptophylla were selected for analysis.Volatile compounds in wood powder samples were detected by headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry(HS-SPME-GC-MS).Their chemical fingerprint information was comprehensively compared and analyzed.Differential compounds were first screened by orthogonal partial least squares discriminant analysis(OPLS-DA),and then partial least squares discriminant analysis(PLS-DA)was used to construct discrimination models for the four wood species.Finally,chemical markers for species identification were determined.The results showed marked differences in the volatile chemical profiles among the four species.P.hui and P.bournei were mainly characterized by terpenoids,whereas M.pauhoi and M.leptophylla were dominated by acids,ketones,and aldehydes.The differential compounds identified by OPLS-DA mainly included terpenoids,esters,heterocyclic compounds,and ketones,and the differences were more pronounced in intergeneric comparisons.The PLS-DA discrimination model achieved an overall identification accuracy of 95.24%.Further screening identified 428 significantly different compounds,mainly esters(15.65%),terpenoids(14.95%),ketones(13.55%),and heterocyclic compounds(12.62%),which together accounted for the major chemical differences among species.Based on variable importance in projection(VIP)values,the top 20 significantly differential compounds were selected as chemical markers,including 2-[(2S,4aR,8aR)-4a,8-dimethyl-2,3,4,5,6,8a-hexahydro-1H-naphthalen-2-yl]propan-2-ol,2-methylnaphthalene,naphthalene,2,6-dimethylthiophenol,2-[(2S,4aR)-4a,8-dimethyl-2,3,4,5,6,7-hexahydro-1H-naphthalen-2-yl]propan-2-ol,and 2-piperidinimine.Heatmap analysis further showed that these 20 chemical markers could reliably distinguish the four wood species.Therefore,HS-SPME-GC-MS can be used to achieve species-level identification of Phoebe and Machilus wood.These results provide a scientific basis for the rational utilization and standardized market management of Phoebe and Machilus wood resources,and also offer practical support for wood identification based on volatile compound chemical fingerprints.关键词
木材树种识别/楠属/润楠属/挥发性物质/化学指纹/顶空固相微萃取-气相色谱-质谱联用技术Key words
wood identification/Phoebe/Machilus/volatile compounds/chemical fingerprint/HS-SPME-GC-MS分类
农业科技引用本文复制引用
罗莎,于占江,吴义强..基于挥发性物质化学指纹方法识别楠属和润楠属木材研究[J].木材科学与技术,2026,40(1):27-40,14.基金项目
国家科技基础资源调查专项项目"《中国木材志》修编"(2023FY101402). （2023FY101402）
