南京中医药大学学报2026，Vol.42Issue(4)：510-522,13.DOI:10.14148/j.issn.1672-0482.2026.0510
基于多组学技术分析射血分数保留的心力衰竭气虚血瘀证的生物学特征
Multi-Omics Analysis of Biological Characteristics in HFpEF with Qi Deficiency and Blood Stasis Syndrome
摘要
Abstract
OBJECTIVE To elucidate the multi-omics molecular characteristics of heart failure with preserved ejection fraction(HFpEF)patients with qi deficiency and blood stasis syndrome,providing a scientific foundation for individualized traditional Chinese medicine(TCM)interventions.METHODS Between July 2022 and June 2023,27 HFpEF patients with qi deficiency and blood sta-sis syndrome and 22 disease controls were enrolled.Metabolomics analysis was performed on peripheral blood plasma samples from all subjects using liquid chromatography-tandem mass spectrometry(LC-MS/MS)to screen for differentially expressed metabolic sub-stances between groups.Transcriptomic sequencing(Bulk RNA-seq)and tandem mass tag(TMT)labeling quantitative proteomic analysis were employed to analyze peripheral blood leukocyte samples from 7 patients with HFpEF of qi deficiency and blood stasis syn-drome and 7 disease controls,identifying differentially expressed genes(DEGs)and differentially expressed proteins(DEPs)between the two groups,followed by bioinformatics analysis.RESULTS Metabolomics results showed that 222 DEGs were screened and 36 were identified between the HFpEF qi deficiency and blood stasis syndrome group and the disease control group.These DEGs were mainly enriched in glycine/serine threonine metabolism,the tricarboxylic acid cycle,and pyruvate metabolism pathways,suggesting that HFpEF patients have amino acid and fatty acid metabolism disorders and impaired energy metabolism.Transcriptomic analysis identified 6 433 DEGs,enriched in biological processes such as inflammatory response,oxidative stress,autophagy,phosphorylation,calcium signaling pathway,and cardiac function.Proteomic analysis revealed 1 124 DEPs,associated with neutrophil extracellular trap formation,the HIF-1 signaling pathway,and metabolic pathways.Multi-omics integration analysis showed that Ca²⁺/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ)and the HIF-1 signaling pathway are key molecular hubs.CONCLUSION HFpEF patients with qi deficiency and blood stasis syndrome exhibit distinct multi-omics alterations characterized by hypoxia,inflammation,au-tophagy,and metabolic dysfunction.Dysregulation of the HIF-1 signaling pathway and upregulation of CaMKII may be potential thera-peutic targets.关键词
射血分数保留的心力衰竭/气虚血瘀证/多组学分析/系统生物学/能量代谢/HIF-1通路/CaMKⅡKey words
heart failure with preserved ejection fraction/qi deficiency and blood stasis syndrome/multi-omics analysis/systems biology/energy metabolism/HIF-1 pathway/CaMKⅡ分类
医药卫生引用本文复制引用
邓帅帅,罗小非,姜国旺,林静怡,赵志强,樊官伟..基于多组学技术分析射血分数保留的心力衰竭气虚血瘀证的生物学特征[J].南京中医药大学学报,2026,42(4):510-522,13.基金项目
国家自然科学基金面上项目(82274436) （82274436）
国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTDC-202207) （ZYYCXTDC-202207）
