宁夏农林科技2026，Vol.67Issue(3)：41-46,6.DOI:10.3969/j.issn.1002-204x.2026.03.007
羊边界病毒纳米PCR检测方法的建立
Establishment of Nano-PCR Detection Method for Border Disease Virus
摘要
Abstract
[Objective]The aim is to develop a rapid detection method for border disease virus(BDV).[Method]Specific primers were designed based on the conserved 5'UTR sequence of BDV.By optimizing the reaction system parameters(including annealing temperature,primer concentration,and nanoparticle addition ratio),a BDV Nano-PCR detection method was successfully constructed.The sensitivity,specificity,and clinical applicability of this method were compared and analyzed and a parallel verification was conducted with those of the conventional PCR method.[Result]The optimized Nano-PCR could stably amplify a BDV-specific target of 210 bp,with a minimum detection limit of 1.1×10 copies/μL,and its sensitivity was 100 times higher than that of the conventional PCR(1.1×103 copies/μL).In the specific detection,this method showed no cross-reaction with bovine viral diarrhea virus,peste des petits ruminants virus,and Caprine enterovirus.The detection of 50 clinical samples showed that the positive detection rate of Nano-PCR was 6%(3/50),which was completely consistent with the results of the conventional PCR,but the sensitivity was significantly higher than that of the conventional PCR.[Conclusion]the established BDV Nano-PCR detection method has higher sensitivity and specificity than the conventional PCR,and can provide technical support for the early diagnosis and epidemiological monitoring of border disease virus infections.关键词
羊边界病毒/纳米PCR/5′UTR基因/临床诊断Key words
Border disease virus/Nano-PCR/5′UTR gene/Clinical diagnosis分类
农业科技引用本文复制引用
于有利,王建东,杨玉秋,周攀..羊边界病毒纳米PCR检测方法的建立[J].宁夏农林科技,2026,67(3):41-46,6.基金项目
宁夏回族自治区中央引导地方科技发展专项(2024FRD05072). （2024FRD05072）
