农业生物技术学报2026，Vol.34Issue(5)：1118-1131,14.DOI:10.3969/j.issn.1674-7968.2026.05.016
靶向普通马铃薯疮痂病的拮抗链霉菌筛选、复配组合及防治效果
Strain Screening,Combinations Preparation and Biocontrol Efficacy of Antagonistic Streptomyces spp.Targeting Potato Common Scab
摘要
Abstract
Potato(Solanum tuberosum)common scab,caused by pathogenic Streptomyces spp.,is an important soil-borne disease that occurs worldwide.Non-pathogenic Streptomyces can produce a large number of metabolites with antimicrobial and growth-promoting effects,making them one of the microorganisms with the greatest biocontrol potential.To obtain non-pathogenic Streptomyces strains with biocontrol potential against Streptomyces scabies which causes potato common scab,this study conducted series of assays,including screening for antimicrobial activity of single strain in vitro,preparation for antagonistic Streptomyces combinations,testing for antimicrobial activity of the combinations,molecular identification of strains included in the different combinations,analysis for their antimicrobial and growth-promoting characteristics,and evaluation for biocontrol efficacy in greenhouse.The results showed that 15 strains with significant antagonistic effects against the S.scabies strain LBX-7 were obtained from 77 non-pathogenic Streptomyces strains.Among them,strain No.ZL21 exhibited the widest inhibition band(8.17 mm).After strain compatibility testing and combinations preparation,3 combinations with antagonistic effects against LBX-7 were obtained.Among these,combination No.C(ZL21\ZL109\ZL144)showed the best antimicrobial effect,with an inhibition band of 7.67 mm in width.Multi-gene sequencing were used to identify the strains in the 3 combinations,strains No.ZL21 and ZL144 belonged to S.olivaceus,while strainsZL54,ZL68,ZL79,ZL90,ZL109 and 05B3 were identified as S.parvus.All strains in the combinations had the abilities to produce protease and chitinase,decompose potassium and fix nitrogen,but none of them could dissolve inorganic phosphorus.Several strains were capable of producing indole-3-acetic acid,cellulase,laccase and amylase.Greenhouse pot control experiment indicated that ZL21 exhibited the highest biocontrol efficacy(75.2%)in all the tested single strains,and combination No.C showed the best efficacy(54.4%)among the 3 combinations in greenhouse.This study obtained candidate Streptomyces strains ZL21 with good biocontrol efficacy against potato common scab.This strain exhibits promising potential as resources for controlling potato common scab.关键词
生防链霉菌/马铃薯疮痂病/抑菌活性/促生能力/生防效果Key words
Biocontrol Streptomyces/Potato common scab/Antimicrobial activity/Growth-promoting ability/Biocontrol efficacy分类
农业科技引用本文复制引用
陈辰,张才华,王世一,张昊玉,丁毓,雷博超,孙蔓莉,赵伟全,于秀梅..靶向普通马铃薯疮痂病的拮抗链霉菌筛选、复配组合及防治效果[J].农业生物技术学报,2026,34(5):1118-1131,14.基金项目
河北省中央引导地方科技发展资金(246Z6503G) （246Z6503G）
河北省自然科学基金(C2025204083) （C2025204083）
石家庄市驻冀高校基础研究项目(241791087A) （241791087A）
