四川林业科技2026，Vol.47Issue(2)：1-7,7.DOI:10.12172/202511120002
大花序桉组培苗增殖培养过程中内源激素变化分析
Analysis of endogenous hormone changes during proliferation of Eucalyptus cloeziana plantlets in tissue culture
摘要
Abstract
Eucalyptus cloeziana,known as'Australian rosewood',is a valuable timber species widely cultivated in southern China.Its rapid growth and high-quality wood make it a key species for national timber reserves.This study aimed to reveal the dynamic changes of endogenous hormones during proliferation of E.cloeziana tissue culture seedlings,in order to provide insights for optimizing clone-specific proliferation systems.Tissue culture seedlings of three E.cloeziana clones(75-23,YD-9,N1)were used as materials.The contents of IAA,ABA,CTK,and GA3 in four parts(cluster buds,leaves,upper stem segments,and lower stem segments)were determined by enzyme-linked immunosorbent assay(ELISA).Statistical analyses,including ANOVA and Duncan's multiple comparisons,were employed to assess distribution characteristics,dynamic changes,and regulatory effects of hormone ratios.The results showed that:(1)Hormone distribution within the same clone exhibited tissue specificity:GA3 content was the highest(1 000-1 500 pg/g)with no significant differences among parts,while CTK content was the lowest(100-250 pg/g)and remained stable.The high-content areas of IAA and ABA varied by clone;for example,in clone 75-23,IAA and ABA were significantly enriched in the upper stem segments,whereas in YD-9 and N1,IAA distribution was uniform,and ABA was significantly higher in stem segments than in leaves and cluster buds.(2)Significant differences in hormone content and composition were observed among clones:the total endogenous hormone content ranked as N1>YD-9>75-23,and the hormone composition for all clones was GA3>ABA>IAA>CTK.(3)During proliferation,hormone contents and ratios changed significantly:as cluster buds developed into stem segments and leaves,hormone contents overall increased,with clone N1 exhibiting the largest increases in IAA(55.8%)and ABA(85.7%).The IAA/CTK ratio increased(e.g.,from 2.5 to 4.5 in N1),while the GA3/ABA ratio decreased(e.g.,from 7.2 to 5.8 in N1),and clonal differences were mainly evident in the late differentiation stage.This study systematically elucidated,for the first time,the distribution and dynamics of endogenous hormones in the proliferation of E.cloeziana tissue culture seedlings,offering a theoretical basis for developing clone-specific proliferation protocols and enhancing seedling efficiency.关键词
大花序桉/组培/无性系/内源激素/增殖/生根Key words
Eucalyptus cloeziana/tissue culture/clone/endogenous hormone/proliferation/rooting引用本文复制引用
黄振,杨勇智,钟星月,刘欣,陈炙,郭敏佳,李佳蔓,郭洪英..大花序桉组培苗增殖培养过程中内源激素变化分析[J].四川林业科技,2026,47(2):1-7,7.基金项目
四川省科技计划项目(2022YFN0003)、四川省林草科技创新团队(2025LCTD0202)资助 （2022YFN0003）
