四川林业科技2026，Vol.47Issue(2)：8-13,6.DOI:10.12172/202510260001
大花序桉组培增殖苗衰退规律及复幼研究
Decline pattern and rejuvenation of proliferating Eucalyptus cloeziana plantlets in tissue culture
摘要
Abstract
To clarify the decline characteristics of tissue culture proliferative seedlings of Eucalyptus cloeziana during continuous subculture and establish an efficient rejuvenation technology system,10 Chuanlin series clones were selected as materials in this study.The dynamic changes of multiplication coefficient,seedling morphology and abnormal traits during the subculture process were systematically monitored,and the formula of rejuvenation and rooting was optimized with the typical decling clone"Chuanlin 73-86"as the model.The results showed that although there were differences in the number of proliferation decline generations among different clones,the change law was consistent:the proliferation ability significantly decreased after 15 consecutive subcultures.For"Chuanlin 73-86",the optimal rejuvenation medium was WPM+0.2 mg/L 6-BA+0.2 mg/L NAA+0.2 mg/L GA3+30 g/L sucrose,with an average multiplication coefficient of 3.17.After rooting,the rejuvenated seedlings had roots with dense root hairs and strong toughness;the seedlings grew vigorously,and the seedling quality met the standard.The decline law revealed and the rejuvenation technology established in this study provide key technical support for the large-scale and stable production of Eucalyptus cloeziana tissue culture seedlings.关键词
大花序桉/无性系/老化/复幼/增殖/生根Key words
Eucalyptus cloeziana/clone/aging/rejuvenation/proliferation/rooting引用本文复制引用
黄振,杨勇智,陈炙,李佳蔓,郭敏佳,赵桃娟,郭洪英..大花序桉组培增殖苗衰退规律及复幼研究[J].四川林业科技,2026,47(2):8-13,6.基金项目
四川省科技计划项目(2022YFN0003)资助 （2022YFN0003）
