四川农业大学学报2026，Vol.44Issue(2)：447-454,8.DOI:10.16036/j.issn.1000-2650.202509650
有色玉米籽粒花青素成分解析及分类方法建立
Analysis of Anthocyanin Components and Establishment of Classification Method for Pigmented Maize Kernels
摘要
Abstract
[Objective]This study aims to establish a classification system for pigmented maize kernels and develop a quantitative classification framework to improve the efficiency of anthocyanin detection and the accuracy of variety screening.[Method]A total of 212 anthocyanin-rich homozygous maize inbred lines were used as materials.Kernel color phenotypes were observed via visual inspection and color cards,while qualitative and quantitative analyses of anthocyanin components were performed using high-performance liquid chromatography(HPLC)to precisely investigate the correlation between color charac-teristics and anthocyanin composition.[Result]The core anthocyanin components in the kernels of pig-mented sweet and waxy maize inbred lines were cyanidin and pelargonidin.The total anthocyanin content in sweet maize was 718.38 mg/kg,which was 1.07 times higher than that in waxy maize.Significant differ-ences were observed between the two types in the detection rate of peonidin and the contribution rate of cyanidin.The anthocyanin content in kernels grown in Yunnan was 8.31%higher than that in Sichuan.A total of 25 germplasm materials with high anthocyanin content were selected,among which the waxy maize line 24CH92 exhibited the highest content at 2,884.09 mg/kg.The 212 inbred lines were classified into 48 groups.The PANTONE 229 U group showed the highest anthocyanin content at 723.43 mg/kg,and RGB values were significantly negatively correlated with anthocyanin content.[Conclusion]An effi-cient and low-cost prediction system for anthocyanin content was established based on the RGB model of kernel color.The quantitative relationship between kernel color and anthocyanin composition was clari-fied,providing a practical technical pathway for the precision breeding and germplasm innovation of pig-mented fresh-eating maize.关键词
鲜食玉米/籽粒花青素/RGB比色卡/高效液相色谱Key words
fresh corn/grain anthocyanins/RGB color chart/HPLC分类
农业科技引用本文复制引用
梅玉芹,蒲汝民,杨国明,周文皓,杨永华,卢艳丽,林海建,李继章..有色玉米籽粒花青素成分解析及分类方法建立[J].四川农业大学学报,2026,44(2):447-454,8.基金项目
云南省科技人才与平台计划(云南省卢艳丽专家工作站)(202305AF150106). （云南省卢艳丽专家工作站）
